宫颈癌患者HPV感染与阴道内环境和血清miRNA表达的相关性分析  

Correlation analysis between HPV infection,vaginal environment,and serum miRNA expression in patients with cervical cancer

作　　者：王海颖 张艳敏 苏佳 杨宝芹 WANG Haiying;ZHANG Yanmin;SU Jia;YANG Baoqin(Inspection Center,Henan Provincial Hospital of Traditional Chinese Medicine(The Second Affiliated Hospital of Henan University of Chinese Medicine),Zhengzhou 450053,China)

机构地区：[1]河南省中医院(河南中医药大学第二附属医院)检验中心,郑州450053

出　　处：《中国医科大学学报》2024年第12期1088-1093,共6页Journal of China Medical University

基　　金：河南省中医药科学研究专项课题(20-21ZY2071)。

摘　　要：目的探讨宫颈癌患者人乳头瘤病毒(HPV)感染与阴道内环境和血清微RNA(miRNA)表达的相关性。方法选取2020年10月至2023年6月我院收治的100例宫颈癌患者作为研究对象,并根据宫颈癌国际妇产科联盟(FIGO)分期分为FIGOⅠB期组(38例)和FIGOⅡA期组(62例),另选取同期宫颈上皮内瘤变(CIN)患者80例作为癌前病变组,比较3组不同病理特征患者阴道内环境微生态因子阳性率及血清miRNA的表达水平。结果FIGOⅡA期组高危型HPV感染率较FIGOⅠB期组和癌前病变组高(P<0.05),FIGOⅡA期组HPV病毒负荷量1级比例低于FIGOⅠB期组和癌前病变组(P<0.05);FIGOⅡA期组过氧化氢(H_(2)O_(2))、唾液酸苷酶(SNA)、pH值、白细胞脂酶(LE)阳性率高于FIGOⅠB期组和癌前病变组(P<0.05);FIGOⅡA期组血清miR-3607-3p、miR-497-5p水平低于FIGOⅠB期组和癌前病变组,血清miR-501水平高于FIGOⅠB期组和癌前病变组(P<0.05);H_(2)O_(2)、SNA、pH值、LE、血清miR-3607-3p、miR-497-5p、miR-501表达水平在不同病理特征中比较,差异有统计学意义(P<0.05)。结论宫颈癌患者肿瘤分化、HPV病毒负荷量、淋巴结转移、FIGO分期与H_(2)O_(2)、SNA、pH值、LE、血清miR-3607-3p、miR-497-5p和miR-501水平密切相关。Objective To explore the correlation between human papilloma virus(HPV)infection and the vaginal environment as well as serum microRNA(miRNA)expression in patients with cervical cancer.Methods A total of 100 patients with cervical cancer admitted to our hospital between October 2020 and June 2023 were enrolled and categorized into two groups:FIGO stageⅠB(38 patients)and FIGO stageⅡA(62 patients).Additionally,80 patients diagnosed with cervical intraepithelial neoplasia(CIN)during the same period were included as the precancerous lesion group.Positive rates of vaginal microenvironmental factors and serum miRNA expression levels were compared across the three groups,considering their different pathological characteristics.Results The high-risk HPV infection rate was significantly higher in the FIGO stageⅡA group compared to both the FIGO stageⅠB and precancerous lesion groups(P<0.05).The proportion of patients with HPV viral load level 1 was significantly lower in the FIGO stageⅡA group than in the FIGO stageⅠB and precancerous lesion groups(P<0.05).The positivity rates of H_(2)O_(2),SNA,pH,and LE in the FIGO stageⅡA group were higher than those in the FIGO stageⅠB and precancerous lesion groups(P<0.05).Serum miR-3607-3p and miR-497-5p levels in the FIGOⅡA stage group were lower than those in the FIGO stageⅠB and precancerous lesion groups,whereas serum miR-501 levels were higher compared with FIGO stageⅠB and precancerous lesion groups(P<0.05).Significant differences in the expression levels of H_(2)O_(2),SNA,pH,LE,serum miR-3607-3p,miR-497-5p,and miR-501 were observed across groups with varying pathological characteristics(P<0.05).Conclusion Tumor differentiation,HPV viral load,lymph node metastasis,FIGO stage,and levels of H_(2)O_(2),SNA,pH,LE,serum miR-3607-3p,miR-497-5p,and miR-501 were closely correlated in patients with cervical cancer.

关 键 词：人乳头瘤病毒 宫颈癌 过氧化氢 唾液酸苷酶 miR-3607-3p miR-497-5p miR-501 

分 类 号：R737.33[医药卫生—肿瘤]