脑胶质瘤组织GATA4、CTNNB1表达水平及其与患者临床特征、预后的关系  

The expression levels of GATA4 and CTNNB1 in brain glioma tissues and their relationship with clinical features and prognosis of patients

作　　者：周高阳 魏明豪 刘海啸 周亮 刘蒙蒙 王蕾 ZHOU Gaoyang;WEI Minghao;LIU Haixiao;ZHOU Liang;LIU Mengmeng;WANG Lei(Department of Neurosurgery,the Second Affiliated Hospital of Air Force Military Medical University,Xi'an,Shaanxi,China,710038)

机构地区：[1]空军军医大学第二附属医院神经外科,陕西西安710038

出　　处：《分子诊断与治疗杂志》2024年第12期2219-2222,2227,共5页Journal of Molecular Diagnostics and Therapy

基　　金：国自然科学基金项目(82101379)。

摘　　要：目的探讨脑胶质瘤组织GATA结合蛋白4(GATA4)、钙黏蛋白相关蛋白(CTNNB1)表达水平及其与患者临床特征、预后的关系。方法选取2018年1月至2020年12月空军军医大学第二附属医院收治的98例脑胶质瘤患者作为研究对象,将治疗前脑胶质瘤组织标本纳入脑胶质瘤组织组(n=98),对应的癌旁组织标本纳入癌旁组织组(n=98)。采用免疫组化法检测组织中GATA4、CTNNB1表达情况;分析GATA4、CTNNB1表达与脑胶质瘤患者病理特征的关系;采用多因素Cox回归分析探讨患者预后的影响因素。结果脑胶质瘤组织组GATA4、CTNNB1阳性表达率高于癌旁组织组,差异有统计学意义(P<0.05)。KPS评分<70分、肿瘤直径>2 cm、淋巴结转移、WHO分级为Ⅲ~Ⅳ期的脑胶质瘤患者GATA4阳性表达率、CTNNB1阳性表达率高于KPS评分≥70分、肿瘤直径≤2 cm、淋巴结无转移、WHO分级为Ⅰ~Ⅱ级的患者,差异有统计学意义(P<0.05)。对98例脑胶质瘤患者出院后连续随访3年,共42例患者存活,总生存率为42.86%(42/98)。KPS≥70分、肿瘤直径≤2 cm、无淋巴结转移、WHO分级Ⅰ~Ⅱ级、GATA4阴性、CTNNB1阴性患者的3年总生存率分别高于KPS<70分、肿瘤直径>2 cm、淋巴结转移、WHO分级Ⅲ~Ⅳ级、GATA4阳性、CTNNB1阳性患者,差异有统计学意义(P<0.05)。多因素Cox回归分析显示:KPS评分<70分,WHO分级Ⅲ~Ⅳ级,GATA4阳性,CTNNB1阳性是影响脑胶质瘤患者预后的因素(P<0.05)。结论GGATA4和CTNNB1在脑胶质瘤组织中主要呈阳性表达,二者与脑胶质瘤患者临床特征及预后相关,有望成为评估脑胶质瘤患者预后的生物学指标。Objective To investigate the expression levels of GATA binding protein 4(GATA4)and cadherin⁃associated protein(CTNNB1)in brain gliomas and their relationship with clinical features and prognosis of patients.Methods 98 patients with glioma who were admitted to the Second Affiliated Hospital of Air Force Medical University from January 2018 to December 2020 were selected as the research subjects.The glioma tissue specimens before treatment were included in the glioma tissue group(n=98),and the corre⁃sponding adjacent tissue specimens were included in the adjacent tissue group(n=98).Immunohistochemistry was used to detect the expression of GATA4 and CTNNB1 in tissues.The aim was to analyze the relationship between the expression of GATA4 and CTNNB1 and the pathological characteristics of glioma patients.Multi⁃variate Cox regression analysis was used to explore the influencing factors of patient prognosis.Results The positive expression rates of GATA4 and CTNNB1 in the glioma tissue group were higher than those in the adja⁃cent tissue group,and the difference was statistically significant(P<0.05).The positive expression rates of GATA4 and CTNNB1 in glioma patients with a KPS score<70,tumor diameter>2 cm,lymph node metasta⁃sis,and WHO gradeⅢ~Ⅳwere higher than those with a KPS score≥70,tumor diameter≤2 cm,no lymph node metastasis,and WHO gradeⅠ~Ⅱ,and the difference was statistically significant(P<0.05).98 patients with glioma were followed up continuously for 3 years after discharge,and 42 patients survived,with an over⁃all survival rate of 42.86%(42/98).The 3⁃year overall survival rate of patients with a KPS≥70 score,tumor diameter≤2 cm,no lymph node metastasis,WHO gradeⅠtoⅡ,GATA4 negative,and CTNNB1 negative was higher than that of patients with a KPS<70 score,tumor diameter>2 cm,lymph node metastasis,WHO gradeⅢtoⅣ,GATA4 positive,and CTNNB1 positive,respectively,and the difference was statistically sig⁃nificant(P<0.05).KPS score<70,WHO gradeⅢ~Ⅳ,GATA4 positive,CTNNB1 positiv

关 键 词：脑胶质瘤 结合蛋白4 钙黏蛋白相关蛋白 

分 类 号：R739.41[医药卫生—肿瘤]