晚期结肠癌患者血清DAO、TLR9表达水平及与肠道菌群失调、化疗疗效的关系  

Serum levels of DAO and TLR9 expression in patients with advanced colon cancer and their relationship with intestinal flora disturbance and chemotherapy efficacy

作　　者：娄智 王鑫[1] 刘亮[2] 胡晨曦[3] 刘玮萱 吉亚君[1] LUO Zhi;WANG Xin;LIU Liang;HU Chenxi;LIU Weixuan;JI Yajun(Department of Oncologyy,Lianyungang First People's Hospital,Lianyungang,Jiangsu,China,222000;Department of Radiation Oncologyy,Lianyungang First People's Hospital,Lianyungang,Jiangsu,China,222000;Precision Laboratory,Lianyungang First People's Hospital,Lianyungang,Jiangsu,China,222000)

机构地区：[1]连云港市第一人民医院肿瘤内科,江苏连云港222000 [2]连云港市第一人民医院肿瘤放疗科,江苏连云港222000 [3]连云港市第一人民医院肿瘤精准实验室,江苏连云港222000

出　　处：《分子诊断与治疗杂志》2024年第12期2252-2255,2260,共5页Journal of Molecular Diagnostics and Therapy

基　　金：江苏省研究生科研与实践新计划(SJCX23_1415)。

摘　　要：目的探讨晚期结肠癌患者血清二胺氧化酶(DAO)、Toll样受体9(TLR9)表达水平及与肠道菌群失调、化疗疗效的关系。方法选择2023年1月至2023月12月于连云港市第一人民医院化疗的120例晚期结肠癌患者作为研究组,另将123名同期体检健康人员作为对照组。比较两组血清DAO、TLR9水平;收集研究组粪便标本并进行肠道菌群测定,根据肠道菌群失调评价标准分为菌群正常组(n=51)、菌群失调Ⅰ度(n=43)、菌群失调Ⅱ度(n=26)。采用Pearson法分析肠道菌群失调相关性,采用受试者工作特征曲线(ROC)评估诊断化疗疗效。结果研究组血清DAO、TLR9水平均高于对照组,差异有统计学意义(P<0.05)。血清DAO、TLR9表达水平:菌群失调Ⅱ度组>菌群失调Ⅰ度组>肠道正常组,差异有统计学意义(P<0.05)。大肠杆菌、粪球菌数量:菌群失调Ⅱ度组>菌群失调Ⅰ度组>肠道正常组,乳酸杆菌、双歧杆菌数量:菌群失调Ⅱ度组<菌群失调Ⅰ度组<肠道正常组,差异有统计学意义(P<0.05)。晚期结肠癌患者血清DAO、TLR9水平均与大肠杆菌、粪球菌均呈正相关,与乳酸杆菌、双歧杆菌均呈负相关(均P<0.05)。无效组晚期结肠癌患者血清DAO、TLR9水平均高于缓解组,差异有统计学意义(P<0.05)。结论晚期结肠癌患者血清DAO、TLR9水平均升高,且与肠道菌群失调、化疗疗效密切相关,两指标对晚期结肠癌患化疗无具有较好的诊断价值。Objective To investigate serum levels of diamine oxidase(DAO)and Toll⁃like recep⁃tor 9(TLR9)expression in patients with advanced colon cancer and their relationship with intestinal flora dis⁃order and chemotherapy efficacy.Methods A total of 120 cases with advanced colon cancer who received chemotherapy from January 2023 to December 2023 were selected as the study group.Additionally,123 healthy individuals who underwent physical examinations during the same period were chosen as the control group.Serum levels of DAO and TLR9 were measured and compared between the two groups.Fecal samples from the study group were collected to analyze their intestinal flora composition.Based on the assessment crite⁃ria for intestinal flora imbalance,the groups were categorized accordingly.The correlation between intestinal flora imbalance and colon cancer was examined using the Pearson method.The effectiveness of chemotherapy was evaluated through the receiver operating characteristic curve(ROC).Results The levels of DAO and TLR9 in the study group were higher than those in the control group,and the differences were statistically significant(P<0.05).The expression levels of DAO and TLR9 in serum were as follows:flora dysregulationⅡgroup>flora dysregulationⅠgroup>intestinal normal group,the difference was statistically significant(P<0.05).The number of Escherichia coli and fecal coccus:flora disorderⅡgroup>flora disorderⅠgroup>intestinal normal group;the number of lactobacillus and bifidobacterium:flora disorderⅡgroup<flora dis⁃orderⅠgroup<intestinal normal group,the difference was statistically significant(P<0.05).Serum DAO and TLR9 levels in advanced colon cancer patients were positively correlated with Escherichia coli and fecal coccus,and negatively correlated with Lactobacillus and bifidobacterium(P<0.05).The levels of DAO and TLR9 in patients with advanced colon cancer in the ineffective group were higher than those in the remission group,and the difference was statistically significant(P<0.05).Conclusio

关 键 词：晚期结肠癌 二胺氧化酶 TOLL样受体9 肠道菌群失调 

分 类 号：R735.35[医药卫生—肿瘤]