食管鳞状细胞癌组织中CST4、VEGF-A表达与病理特征及预后的关系  

Correlation of CST4 and VEGF-A Expression with Pathological Features and Prognosis in Esophageal Squamous Cell Carcinoma

作　　者：宋正宇 郭嘉仪 张海平[1] 张力为[1] 伊地力斯·阿吾提[1] SONG Zhengyu;GUO Jiayi;ZHANG Haiping(Department of Thoracic Surgery,The First Affiliated Hospital of Xinjiang Medical University,Xinjiang 830054,China)

机构地区：[1]新疆医科大学第一附属医院胸外科、新疆医科大学国家重点实验室,乌鲁木齐830054

出　　处：《医学研究杂志》2024年第11期51-56,共6页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81960498)。

摘　　要：目的 探讨食管鳞状细胞癌(esophageal squamous cell carcinoma, ESCC)中半胱氨酸蛋白酶抑制剂S(cysteine protease inhibitor S,CST4)和血管内皮生长因子-A(vascular endothelial growth factor-A,VEGF-A)的表达情况与临床病理参数的相关性。方法 采用免疫组化法检测50例ESCC患者组织中CST4与VEGF-A的表达,并与病理参数进行相关性分析;结合Enrichr、GEO数据库结果确认两者富集情况及相关性。结果 CST4、VEGF-A在ESCC组织中的阳性表达率明显高于癌旁组织,差异有统计学意义(P<0.05)。不同的淋巴结转移、浸润深度、神经脉管侵犯的ESCC组织中,CST4阳性表达率差异有统计学意义(P<0.05)。不同的淋巴结转移、浸润深度、神经脉管侵犯、病理分期的ESCC组织中,VEGF-A阳性表达率差异有统计学意义(P<0.05)。Kaplan-Meier生存分析结果显示,患者术后生存率与CST4、VEGF-A表达情况、浸润深度、TNM分期及淋巴结转移情况有关(P<0.05)。Spearman相关性分析结果显示,CST4和VEGF-A在ESCC组织中的表达呈正相关(r=0.601,P<0.05)。COX回归分析结果显示,VEGF-A表达水平、CST4表达水平、淋巴结转移、侵犯深度和病理分期是影响患者生存的危险因素(P<0.05);富集分析结果显示,CST4、VEGF-A在细胞迁移和侵袭方面起到协同作用。结论 在ESCC组织中,CST4、VEGF-A的表达高于正常组织,可能共同参与了ESCC的发生、发展及转移的过程,有望成为ESCC治疗及判断预后的重要指标。Objective To investigate the correlation between the expression of cysteine protease inhibitor S(CST4)and vascular endothelial growth factor-A(VEGF-A)in esophageal squamous cell carcinoma(ESCC)and clinical pathological parameters.Methods Immunohistochemistry was used to detect the expression of CST4 and VEGF-A in the tissues of 50 patients with ESCC,and the correlation between them and pathological parameters was analyzed.The enrichment and correlation of them were confirmed by combining the results of Enrichr and GEO databases.Results Compared with adjacent tissues,the positive expression rates of CST4 and VEGF-A in ESCC was significantly increased(P<0.05).There were significant differences in the positive expression rate of CST4 in ESCC with different lymph node metastasis,depth of invasion,and neurovascular invasion(P<0.05).There were significant differences in the positive expression rate of VEGF-A in ESCC with different lymph node metastasis,depth of invasion,neurovascular invasion,and pathological stage(P<0.05).Kaplan-Meier survival analysis indicated that postoperative survival rate of patients was related to the expression of CST4 and VEGF-A,depth of invasion,TNM stage and lymph node metastasis(P<0.05).Spearman correlation analysis showed a positive correlation between the expression of CST4 and VEGF-A in ESCC tissues(r=0.601,P<0.05).COX regression analysis showed that VEGF-A expression level,CST4 expression level,lymph node metastasis,depth of invasion and pathological stage were risk factors for survival(P<0.05).Enrichment analysis showed that CST4 and VEGF-A play a synergistic role in cell migration and invasion.Conclusion CST4 and VEGF-A are higher expressed in ESCC tissues than normal tissues.They are involved in the development and metastasis of ESCC,and they are expected to be important indicators for the treatment and prognosis of ESCC.

关 键 词：食管鳞癌 半胱氨酸蛋白酶抑制剂S 血管内皮生长因子-A 预后 

分 类 号：R735[医药卫生—肿瘤]