miR-198对肝癌细胞株增殖和迁移的影响  

Effects of MiR-198 on Proliferation and Migration of Hepatocellular Carcinoma Cell Lines

作　　者：王磊 韩全乡 李灵招 徐文宁 李醒亚[4] WANG Lei;HAN Quanxiang;LI Lingzhao(Department of Radiation Oncology,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Henan 450003,China)

机构地区：[1]郑州大学附属郑州中心医院放疗科,450003 [2]郑州大学附属郑州中心医院检验科,450003 [3]河南省中医院(河南中医药大学第二附属医院),450011 [4]郑州大学第一附属医院肿瘤科,450003

出　　处：《医学研究杂志》2024年第11期90-94,共5页Journal of Medical Research

基　　金：河南省医学科技攻关计划项目(2018020820);河南省科技攻关计划项目(182102310122)。

摘　　要：目的 探讨miR-198对肝癌细胞株增殖和迁移的影响。方法 采用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction, RT-qPCR)法检测miR-198在肝癌的不同细胞株中的表达量,然后运用细胞实验及动物实验进行miR-198对Huh7细胞株增殖、迁移及成瘤能力的研究。结果 RT-qPCR检测结果显示,与Chang liver和LO2正常肝细胞株比较,HepG2、Huh7、Hep3B和MHCC97H肝癌细胞株中的miR-198表达量降低,其中在Huh7细胞中尤为明显;流式细胞术检测结果显示,miR-198能够促进Huh7细胞凋亡;CCK-8及软琼脂克隆形成实验结果显示,与对照组比较,Huh7肝癌细胞株在转染miR-198模拟物后其增殖能力显著降低;划痕实验及Transwell实验检测结果显示,miR-198能够抑制Huh7肝癌细胞株的迁移能力;裸鼠成瘤实验结果显示,转染miR-198模拟物的Huh7肝癌细胞株的成瘤能力受到明显抑制。结论 miR-198在HepG2、Huh7、Hep3B和MHCC97H肝癌细胞株中的表达量明显降低,且在Huh7肝癌细胞株中最低;miR-198能够抑制Huh7肝癌细胞株增殖及迁移能力,降低其成瘤功能。Objective To investigate the effects of miR-198 on proliferation and migration of hepatocellular carcinoma cell lines.Methods RT-qPCR was used to detect the expression of miR-198 in different hepatocellular carcinoma cell lines,and the proliferation,migration and tumorigenic ability of miR-198 on Huh7 cell lines were identified by in cell experiments and in animal experiments.Results RT-qPCR test results showed that compared with Chang liver and LO2 normal liver cell lines,the expression levels of miR-198 in HepG2,Huh7,Hep3B and MHCC97H hepatocellular carcinoma cell lines were decreased,especially in Huh7 cells.Flow cytometry was used to confirm that miR-198 promoted the apoptosis of Huh7 cells.CCK-8 and soft agar clone formation experiment showed that compared with the control group,the proliferation of Huh7 hepatocellular carcinoma cell line after transfection with miR-198mimics was significantly reduced.The results of scratch test and Transwell test showed that miR-198 could inhibit the migration ability of Huh7 hepatocellular carcinoma cell line.The tumor formation experiment in nude mice proved that the tumor-forming ability of Huh7 hepatocellular carcinoma cell line transfected with miR-198 was obviously inhibited.Conclusion The expression level of miR-198 in HepG2,Huh7,Hep3B and MHCC97H hepatocellular carcinoma cell lines was significantly decreased,and the lowest expression was found in Huh7 hepatocellular carcinoma cell line.MiR-198 can inhibit the proliferation and migration ability of Huh7 hepatocellular carcinoma cell line,and reduce its tumor-forming ability.

关 键 词：miR-198 肝癌细胞株 增殖 迁移 

分 类 号：R735[医药卫生—肿瘤]