LncRNA SNHG1通过miR-361-3p/Nfat5信号通路对阿尔茨海默病细胞模型损伤的影响  

Effect of LncRNA SNHG1 on the Damage of Alzheimer's Disease Cell Models through the miR-361-3p/Nfat5 Signaling Pathway

作　　者：刘丽丹[1] 徐佳骏[1] 刘文萍[1] 李达丽[1] 彭颜晖[1] LIU Li-dan;XU Jia-jun;LIU Wen-ping;LI Da-li;PENG Yan-hui(Department of Neurology,The Sixth Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang,830002,China)

机构地区：[1]新疆医科大学第六附属医院神经内科,新疆乌鲁木齐830002

出　　处：《现代生物医学进展》2024年第22期4221-4223,4284,共4页Progress in Modern Biomedicine

基　　金：新疆维吾尔自治区自然科学基金项目(2021D01C456)。

摘　　要：目的:探讨LncRNA SNHG1表达对AD细胞损伤模型的影响,并研究其作用与miR-361-3p/Nfat5通路的关系。方法:建立AD细胞模型。向SH-SY5Y细胞转入SNHG1-shRNA以减低SNHG1表达,转入miR-361-3p-mimic以过表达miR-361-3p。检测SNHG1和miR-361表达水平、细胞凋亡、蛋白表达水平,并验证miR-361-3p与SNHG1结合。结果:与空白对照组相比,AD模型组细胞miR-361-3p表达水平降低,而SNHG1表达水平升高,细胞凋亡率升高(P<0.05)。敲低SNHG1表达降低可降低细胞凋亡率(P<0.05)。过表达miR-361-3p降低SNHG1表达水平。AD模型组细胞Nfat5和p-Tau蛋白表达均升高,而Nestin蛋白表达降低(P<0.05);敲低SNHG1降降低AD细胞模型中Nfat5和p-Tau蛋白表达水平,提高Nestin蛋白表达水平。结论:敲低SNHG1可降低Aβ25-35蛋白诱导的AD细胞模型损伤,其机制与激活miR-361-3p/Nfat5通路有关。Objective:To investigate the effect of LncRNA SNHG1 expression on AD cell damage model,and to study its relationship with miR-361-3p/Nfat5 pathway.Methods:Establish an AD cell model.To SH-SY 5 Y cells to SNHG 1-shRNA to reduce SNHG 1 expression and to miR-361-3p-mimic to overexpress miR-361-3p.The expression levels of SNHG 1 and miR-361,apoptosis,and protein expression were examined,and miR-361-3p was bound to SNHG 1.Results:Compared with the blank control group,the AD model group showed decreased miR-361-3p expression,but increased SNHG 1 expression and increased apoptosis rate(P<0.05).Knockdown of reduced SNHG 1 expression decreased the apoptosis rate(P<0.05).Overexpression of miR-361-3p reduces the level of SNHG 1 expression.In the AD model group,Nfat 5 and p-Tau were increased,while Nestin protein decreased(P<0.05);knockdown of SNHG 1 decreased the expression of Nfat 5 and p-Tau and increased the Nestin protein expression.Conclusion:Knockdown of SNHG1 can reduce the damage induced by Aβ25-35 protein in AD cell models,and the mechanism is related to the activation of miR-361-3p/Nfat5 pathway.

关 键 词：LncRNA SNHG1 miR-361-3p 阿尔茨海默症 凋亡 

分 类 号：R-33[医药卫生] R749.16