低强度视网膜超声刺激对视觉皮层神经活动的调节作用研究  

Study on the regulatory effect of low intensity retinal ultrasound stimulation on the neural activity of visual cortex

作　　者：王倩倩 袁毅[1,2] 闫佳庆[3] WANG Qianqian;YUAN Yi;YAN Jiaqing(School of Electrical Engineering,Yanshan University,Qinhuangdao,Hebei 066004,P.R.China;Key Laboratory of Intelligent Rehabilitation and Neuromodulation of Hebei Province,Yanshan University,Qinhuangdao,Hebei 066004,P.R.China;College of Electrical and Control Engineering,North China University of Technology,Beijing 100041,P.R.China)

机构地区：[1]燕山大学电气工程学院,河北秦皇岛066004 [2]燕山大学河北省智能康复及神经调控重点实验室,河北秦皇岛066004 [3]北方工业大学电气与控制工程学院,北京100041

出　　处：《生物医学工程学杂志》2024年第6期1161-1168,共8页Journal of Biomedical Engineering

基　　金：河北省自然科学基金(F2022203050);国家自然科学基金(62273291)。

摘　　要：低强度超声刺激视网膜具有对初级视觉皮层(V1)的神经活动进行调节的能力,但目前尚不明确不同强度和时间的超声作用视网膜时,如何调节V1内神经活动。在本文中,记录了不同超声强度和不同时间下小鼠V1脑区的局部场电位(LFP)信号,分析了超声刺激前1 s至刺激后2 s(–1~2 s)对应的LFP幅值,以及delta、theta、alpha、beta、低gamma频段的功率和样本熵。实验结果表明低强度超声刺激视网膜时,随着刺激强度的增加,视觉皮层局部场电位峰峰值呈现线性上升趋势;delta、theta频段的功率呈现线性上升趋势,样本熵呈现线性下降趋势。随着刺激时间的增加,视觉皮层局部场电位峰峰值呈现上升趋势,上升趋势逐步减弱;delta频段的功率呈现上升趋势,样本熵呈现线性上升趋势,而theta频段的样本熵呈现下降趋势。结果表明,低强度视网膜超声刺激对于视觉皮层的神经活动有显著的调节作用。本研究能帮助理解超声刺激对视觉系统功能的调控机制,推动个体化多参数调控治疗视觉神经退化、视网膜病变等疾病研究的开展。Low-intensity ultrasound stimulation of the retina has the ability to modulate neural activity in the primary visual cortex(V1),however,it is currently unclear how different intensities and durations of ultrasonic stimulation of the retina modulate neural activity in V1.In this paper,we recorded local field potential(LFP)signals in the V1 brain region of mice under different ultrasound intensities and different stimulation times.The amplitude of LFP corresponding to 1 s before ultrasound stimulation to 2 s after stimulation(–1–2 s)was analyzed,including the power and sample entropy of delta,theta,alpha beta,and low gamma frequency bands.The experimental results showed that,as the stimulation intensity increased,the peak value of the LFP in the visual cortex showed a linear upward trend;the power in the delta and theta frequency bands showed a linear upward trend,and the sample entropy showed a linear downward trend.With increases of stimulation duration,the peak value of the LFP in the visual cortex showed an upward trend,and the upward trend gradually weakened;the power in the delta frequency band showed an upward trend,the sample entropy showed a linear upward trend,and the sample entropy in the theta frequency band showed a downward trend.The results show that low-intensity ultrasonic stimulation of the retina has a significant modulatory effect on neural activity in the visual cortex.The study provides insights into the mechanisms by which ultrasonic stimulation regulates visual system function.Furthermore,it clarifies the patterns of parameter selection,facilitating the development of personalized multiparameter modulation for the treatment of visual neural degeneration,retinal disorders and related research areas.

关 键 词：低强度超声刺激 视网膜 初级视觉皮层 局部场电位 超声参数 

分 类 号：R774.1[医药卫生—眼科]