2021—2023年南充市临床腹泻标本沙门菌分子分型及耐药性分析  

Molecular typing and drug resistance analysis of Salmonella in clinical diarrhea specimens in Nanchong City from 2021 to 2023

作　　者：张艳[1] 胡康 张帆 ZHANG Yan;HU Kang;ZHANG Fan(Nanchong Center for Disease Control and Prevention,Nanchong 637000,Sichuan Province,China.)

机构地区：[1]南充市疾病预防控制中心,四川南充637000

出　　处：《寄生虫病与感染性疾病》2024年第4期239-245,共7页Parasitoses and Infectious Diseases

摘　　要：目的 了解2021—2023年南充市临床腹泻标本中分离的沙门菌的分子分型及药物敏感性特征,为沙门氏菌病的溯源调查及临床用药提供依据。方法 按照国家致病菌识别网技术规范中的操作程序,对2021—2023年南充市临床腹泻标本中分离出的103株沙门菌进行脉冲电场凝胶实验(pulsed field gel electrophoresis,PFGE),根据革兰阴性药敏检测试剂/盘式说明书测定出菌株对17种药物的最小抑菌浓度(minimum inhibitory concentration,MIC)值,运用WPS 2019软件对耐药情况进行分析,并绘制表格及耐药谱。结果 103株沙门菌PFGE条带经聚类分析得到89种带型,2022年有9株菌为同一种型别。8株沙门菌对17种药物均敏感,其余95株均有不同程度的耐药。其中,对氨苄西林(AMP)的耐药率最高,达到79.61%;其次是四环素(TET),为60.20%。所有沙门菌对阿奇霉素(AZM)均表现为中等耐药或敏感,78株沙门菌存在多重耐药现象。结论 南充市沙门菌分子型别总体存在多样性,多重耐药现象严重,应提前建立防控策略及合理用药方案,降低公共卫生问题发生风险。Objective To understand the molecular typing and drug sensitivity characteristics of Salmonella isolated from clinical diarrhea specimens in Nanchong City from 2021 to 2023, and to provide basis for the traceability investigation and clinical medication of Salmonella disease.Methods According to the operational procedures of the technical specifications of National Pathogen Identification Network,103 strains of Salmonella isolated from clinical diarrhea specimens were subjected to pulsed field gel electrophoresis experiment(PFGE). The minimum inhibitory concentration(MIC) values of the strains for 17 kinds of drugs were determined based on Gramnegative drug sensitivity testing reagents or disk instructions. WPS 2019 software was used to analyze the resistance situation and draw a table and drug resistance spectrum. Results The 103Salmonella PFGE bands were clustered and analyzed, resulting in 89 band types. There were 9strains of the same type in 2022. Eight Salmonella strains were found to be sensitive to the 17kinds of drugs, while the remaining 95 strains had different degrees of resistance. Among them,the resistance rate to ampicillin(AMP) was the highest,reaching 79.61%,followed by tetracycline(TET) at 60.20%. All Salmonella strains showed moderate resistance or sensibility to azithromycin(AZM), and 78 strains exhibited multiple drug resistance. Conclusions There was an overall diversity in the molecular types of Salmonella in Nanchong City,and the phenomenon of multiple drug resistance was severe. Prevention and control strategies and rational drug use plans should be established in advance to reduce the risk of public health problems.

关 键 词：腹泻 沙门菌 脉冲场凝胶电泳 分子分型 耐药性 

分 类 号：R155.3[医药卫生—营养与食品卫生学]