Risk assessment of Bacillus cereus in lettuce grown in open-feld and greenhouse farming systems in China:A comparative study  

作　　者：Yang Qu ShenghaoYu Haotian Gu WenWang WenboWang Zhaoying He Hong Shan Min Gao Xin Zhao ChaoWei TingtingTao Guanghua Zhao Changyan Zhou Yujuan Suo 

机构地区：[1]Laboratory of Quality and Safety Risk Assessment for Agro-Products of Ministry of Agriculture and Rural Affairs,Institute for Agro-Food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences,Shanghai,China [2]Information Application Research Center of Shanghai Municipal Administration for Market Regulation,Shanghai,China [3]Eco-environmental Protection Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,China [4]MOA Laboratory of Quality&Safety Risk Assessment for Agro-products(Hangzhou),Institute of Agro-Product Safety and Nutrition,Zhejiang Academy of Agricultural Sciences,Hangzhou,China [5]Laboratory of Quality and Safety Risk Assessment for Agro-Products of Ministry of Agriculture and Rural Affairs,Institute of Agricultural Standards and Testing Technology for Agri-Products,Shandong Academy of Agricultural Sciences,Jinan,China [6]Institute of Quality Standard and Testing Technology,Beijing Academy of Agriculture and Forestry Sciences,Beijing,China [7]Quality and Safety Institute of Agricultural Products,Heilongjiang Academy of Agricultural Sciences,Harbin,China [8]Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing,China [9]Institute of Germplasm Resources and Biotechnology,Tianjin Academy of Agricultural Sciences,Tianjin,China [10]Laboratory of Quality and Safety Risk Assessment for Agro-Products of Ministry of Agriculture and Rural Affairs,Institute of Quality Standard and Testing Technology for Agro-Products of Sichuan Academy of Agricultural Sciences,Chengdu,China [11]Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality,Institute of Food Safety and Nutrition,Jiangsu Academy of Agricultural Sciences,Nanjing,China [12]Research Institute of Agricultural Quality Standards and Testing Techniques,Henan Academy and Agricultural Sciences,Zhengzhou,China

出　　处：《Food Quality and Safety》2024年第2期268-277,共10页食品品质与安全研究（英文版）

基　　金：supported by the Shanghai Agriculture Applied Technology Development Program,China(No.X20210302);the Shanghai Sailing Program(No.22YF1440800);the Special Project of Agriculture Produce Quality Safety Risk Assessment(Nos.GJFP2019005 and GJFP20220205);from the Ministry of Agriculture the Rural Affairs of the People’s Republic of China,and the Applied Basic Research Project of Shanghai Academy of Agricultural Sciences(CN)(No.202309).

摘　　要：Objectives:Bacillus cereus isolates from vegetables can cause diarrheal syndrome food poisoning.Lettuce,the most highly consumed readyto-eat vegetable,poses a signifcant risk of B.cereus exposure.Hence,B.cereus monitoring and evaluation during cultivation are essential for preventing foodborne illnesses caused by this food-pathogen combination.However,the impact of different cultivation practices on B.cereus contamination in lettuce currently remains unclear.Furthermore,a validity indicator for the experimental models cannot be effectively validated under feld conditions.Materials and Methods:Herein,we assessed the risk of B.cereus exposure using lettuce grown under two different cultivation farming systems in China(open-feld versus greenhouse)using a quantitative microbial risk assessment model.Tukey’s mean comparison method and kernel density estimation frequency distribution statistics were used for model validation using environmental samples and lettuce.Results:The mean concentration of B.cereus in open-feld-grown lettuce was signifcantly higher than that in greenhouse-grown lettuce(P<0.05).No signifcant differences were observed between measured,formula-calculated,and risk assessment values in environmental samples or lettuce(P>0.05),verifying the accuracy of the risk model.Conclusion:Soil solarization,complete manure treatment,usage of mulch flms,and irrigation water quality should be considered in agricultural practices to control B.cereus concentrations in lettuce.Overall,we found that B.cereus was more abundant in open-feld-grown lettuce,and we provide a risk evaluation framework that can guide farmers and policy-makers to control B.cereus contamination in farm-to-fork lettuce chains in China.

关 键 词：Diarrheal syndrome food poisoning ready-to-eat vegetable GREENHOUSE microbial risk assessment 

分 类 号：S472[农业科学—农业昆虫与害虫防治]