Genetic identifcation,pathogenicity and patulin production of Penicillium species from apple blue mold in China  

作　　者：Youming Shen Wenyu Ma Ning Ma Mengyao Li Jianyi Zhang Guofeng Xu 沈友明;马文宇;马宁;李梦瑶;张建一;徐国锋(Laboratory of Quality and Safety Risk Assessment for Fruit(Xingcheng)Ministry of Agriculture and Rural Affairs,Quality Inspection and Test Center for Fruit and Nursery Stocks Ministry of Agriculture and Rural Affairs(Xingcheng),Research Institute of Pomology Chinese Academy of Agricultural Sciences,Xingcheng,China;College of Veterinary Medicine,Agricultural University of Hebei,Baoding,China)

机构地区：[1]Laboratory of Quality and Safety Risk Assessment for Fruit(Xingcheng)Ministry of Agriculture and Rural Affairs,Quality Inspection and Test Center for Fruit and Nursery Stocks Ministry of Agriculture and Rural Affairs(Xingcheng),Research Institute of Pomology Chinese Academy of Agricultural Sciences,Xingcheng,China [2]College of Veterinary Medicine,Agricultural University of Hebei,Baoding,China

出　　处：《Food Quality and Safety》2024年第2期414-426,共13页食品品质与安全研究（英文版）

基　　金：supported by the Youth Innovation Program of Chinese Academy of Agricultural Sciences(No.Y2023QC26);the Agricultural Science and Technology Innovation Program(No.CAAS-ASTIP),China.

摘　　要：Objectives:Blue mold is a common postharvest disease that leads to severe rot and patulin(PAT)contamination in fruits,which is a key factor endangering the storage and quality of apple products.However,the Penicillium species from apple blue mold in China and their pathogenicity and PAT production ability have not been well studied.Materials and Methods:In this study,62 strains of pathogenic Penicillium species were isolated from blue mold apples collected from different regions of China.The ITS,BenA,and CaM genes were sequenced for molecular identifcation.Results:Penicillium strains were identifed as three species,57 P.expansum,3 P.crustosum,and 2 P.polonicum.Twelve variants of the ITS sequence,61 variants of BenA and 67 variants of CiaM genes were identifed between Penicillium species.Fungal morphology was observed on potato dextrose agar(PDA).After 10 d of cultivation,P.expansum cultures were detected with PAT concentrations ranging from 457.98 to 1333.85 mg/kg,and two P.polonicum strains were 6.02 mg/kg and 6.30 mg/kg.The three P.crustosum strains could not produce PAT on PDA.In apple inoculation,P.expansum strains showed signifcantly faster infection rates than P.crustosum and P.polonicum strains.All P.expansum strains can produce PAT during infection,and the concentrations in the rot apple tissues were 2.31-88.67 mg/kg.The P.crustosum and P.polonicum strains could not produce PAT during infection.Conclusions:This study provides data and information regarding the morphology,virulence and PAT production of Penicillium pathogens that will improve the understanding of apple blue mold and postharvest disease control.

关 键 词：Malus domestica blue mold Penicillium expansum single nucleotide polymorphism mycotoxins 

分 类 号：S432.4[农业科学—植物病理学] R155.5[农业科学—农业昆虫与害虫防治]