‘呼红’牡丹高温胁迫响应生理与转录组分析  

Transcriptome Analysis of the Response of Tree Peony‘Hu Hong’Under High Temperature Stress

作　　者：卜文轩 姚奕平 黄宇 杨星宇 罗小宁 张旻桓 雷维群 王政[3] 田珈宁 陈露洁 秦莉萍[2] BU Wenxuan;YAO Yiping;HUANG Yu;YANG Xingyu;LUO Xiaoning;ZHANG Minhuan;LEI Weiqun;WANG Zheng;TIAN Jianing;CHEN Lujie;QIN Liping(College of Landscape Architecture,Central South University of Forestry and Technology,Changsha 410004,China;College of Art and Design,Nanning University,Nanning 530200,China;College of Landscape Architecture and Art,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]中南林业科技大学风景园林学院,长沙410004 [2]南宁学院艺术与设计学院,南宁530200 [3]河南农业大学风景园林与艺术学院,郑州450046

出　　处：《园艺学报》2024年第12期2800-2816,共17页Acta Horticulturae Sinica

基　　金：湖南省自然科学基金项目(2022JJ31010);湖南省自然科学基金青年基金项目(2023JJ41035);湖南省研究生创新基金项目(2023CX02013;CX20240698);中南林业科技大学引进人才科研启动基金项目(2019YJ037);中南林业科技大学研究生科研创新基金项目(2024CX02014);国家自然科学基金青年基金项目(32401639);湖南省林业科技攻关与创新项目(XLK202432);湖南省教育厅重点项目(24A0195)。

摘　　要：为探究中国南方湿热地区本土牡丹(Paeoniasuffruticosa)品种高温胁迫阶段的分子调控机制,以江南牡丹品种‘呼红’2~3年生植株为试材,采集20℃常温对照与40℃高温处理0、2、6、12和24h的成熟叶片,采用生理指标和转录组分析方法探究高温胁迫下不同处理时间基因表达的差异。试验结果表明,随着高温胁迫时间的延长,叶片失水,生物膜受损,相对电导率和脯氨酸含量显著增加,明确了游离脯氨酸、相对电导率与可溶性蛋白为衡量牡丹高温胁迫响应中生理机制变化的重要指标。转录组测序总计产生492693个unigene和840509个转录本,36174条差异表达基因在GO、KEGG等5个数据库得到注释。GO富集分析表明,高温处理对比组中热的反应途径、还原型戊糖磷酸循环途径等是主要的富集途径。KEGG富集分析表明,参与信号传导、光合作用、新陈代谢等相关的差异表达基因在响应高温胁迫中起主要作用。通过转录组分析,挖掘到多个参与耐热胁迫响应的转录因子和重要基因,包括热激转录因子PsHSFA2b、PsHSFA3、PsHSFB2b/2a、PsHSFB4、PsHSFC1和NAC、MYB、bHLH等家族基因。对选取的11个基因通过qRT-PCR检测在高温胁迫下的表达,其结果与RNA-seq结果相似。To analyze the molecular regulation mechanism of tree peony(Paeonia suffruticosa)in southern China,‘Hu Hong’was selected as the test material,the leaves treated at 20℃and 40℃were collected after 0,2,6,12 and 24 h,respectively.Transcriptomics and physiological and biochemical indicators were used to explore the differences in gene expression at different treatment times under high temperature stress.The result showed that the leaves showed water loss and biofilm damage,the relative conductivity of leaves and the proline content increased significantly with the prolongation of high temperature stress time.Free proline,relative conductivity and soluble protein were identified as important indicators to measure the changes of physiological mechanism in the response of peony to high temperature stress.A total of 492693 unigene and 840509 transcripts were generated by RNA-seq.36174 differentially expressed genes(DEGs)were annotated in 5 databases including GO and KEGG.The GO analysis showed that the main enrichment pathways were the reaction pathway to heat and the cycle pathway of reduced pentose phosphate.KEGG enrichment analysis showed that DEG is involved in signal transduction,photosynthesis and metabolism play a major role in responding to high temperature stress.Among the DEGs,multiple candidate genes and transcription factors involved in heat stress have been screened out,such as heat shock transcription factors PSHSFA2b,PSHSFA3,PSHSFB2a,PSHSFB2b,PSHSFB4,PSHSFC1 and some NAC,MYB,bHLH family genes.The expression of 11 selected genes under high temperature stress was detected by qRT-PCR,and the results were similar to RNA seq.

关 键 词：牡丹 高温胁迫 转录组 生理生化指标 

分 类 号：S685.11[农业科学—观赏园艺]