糙皮侧耳乳酸脱氢酶对低温光照下菌丝生长的调控作用  

Study on the Regulation of Lactate Dehydrogenase on Mycelia Growth of Pleurotus ostreatus Under Low Temperature and Light

作　　者：吴柏增 降玉清 何琪 黄晨阳[1] 张利姣 赵梦然[1] WU Baizeng;JIANG Yuqing;HE Qi;HUANG Chenyang;ZHANG Lijiao;ZHAO Mengran(National Key Laboratory for Efficient Utilization of Arid and Semi-Arid Cultivated Land in North China,Key Laboratory of Microbial Resources Collection and Preservation,Ministry of Agriculture and Rural Affairs,Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Horticulture,Jilin Agriculture University,Changchun 130118,China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]中国农业科学院农业资源与农业区划研究所,农业农村部农业微生物资源收集与保藏重点实验室,北方干旱半干旱耕地高效利用全国重点实验室,北京100081 [2]吉林农业大学园艺学院,长春130118 [3]吉林农业大学食药用菌教育部工程研究中心,长春130118

出　　处：《园艺学报》2024年第12期2886-2894,共9页Acta Horticulturae Sinica

基　　金：国家自然科学基金青年基金项目(32002110,32302638);现代农业产业技术体系建设专项资助(CARS-20)。

摘　　要：为探索乳酸脱氢酶(lactate dehydrogenase,LDH)在低温光照刺激下对糙皮侧耳(Pleurotus ostreatus)菌丝生长的影响,检测了低温光照下乳酸脱氢酶的表达特征,观测了外源添加乳酸脱氢酶抑制剂、乳酸、丙酮酸对菌丝生长的影响。结果表明,乳酸脱氢酶对15℃、光照12 h/黑暗12 h条件下的菌丝生长具有促进作用。进一步研究发现,抑制乳酸脱氢酶活性会导致丙酮酸脱氢酶(pyruvate dehydrogenase,PDH)活性下降15.02%,进入三羧酸循环的丙酮酸通量减少,ATP产生量降低14.78%,胞内乳酸和丙酮酸含量分别增加18.97%和12.57%;乳酸和丙酮酸的积累能够诱导乙醇脱氢酶(alcohol dehydrogenase,ADH)活性升高,胞质NAD+含量增加,NADH/NAD~+比例失衡,从而抑制菌丝生长。In order to study the effect of lactate dehydrogenase on mycelial growth of Pleurotus ostreatus under the environmental stimulation of low temperature and light,the expression profiles of lactate dehydrogenase were analyzed,and the influences of exogenous lactate dehydrogenase inhibitor,lactic acid,and pyruvic acid on the mycelial growth were observed.The results showed that lactate dehydrogenase could accelerate mycelial growth in 12 h of light alternated with 12 h of darkness at 15℃.Inhibition of lactate dehydrogenase enzyme activity would cause the activity of pyruvate dehydrogenase(PDH)to be decreased by 15.02%,which giving rise to the decreasing flux of pyruvic acid into the tricarboxylic acid cycle,the production of ATP reduced by 14.78%,and the intracellular contents of lactic acid and pyruvic acid increased by 18.97%and 12.57%,respectively.The accumulation of lactic acid and pyruvic acid could induce increased activities of alcohol dehydrogenase,which leading to the increase of NAD+content and the imbalance of NADH/NAD+ratio,and therefore the inhibition of mycelial growth.

关 键 词：糙皮侧耳 乳酸脱氢酶 乳酸 糖代谢 低温 光照 

分 类 号：S646.141[农业科学—蔬菜学]