Effects of acupuncture on serotonin,histamine,substance P,and tryptase levels at sensitized points in model rats with knee osteoarthritis  

作　　者：Jiayi Yang Zidong Wang Jing Jiang Huiling Tian Shun Wang Yizhi Liu Zumao Cao Changqing Joseph Yang Zhigang Li 

机构地区：[1]School of Acupuncture,Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing,102488,China [2]School of Nursing,Beijing University of Chinese Medicine,Beijing,102488,China [3]School of Traditional Chinese Medicine,Capital Medical University,Beijing,100050,China [4]Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing,100029,China [5]Dongzhimen Hospital Key Laboratory,Beijing University of Chinese Medicine,Beijing,100700,China [6]American Institute of Mental Health in Traditional Chinese Medicine,Los Angeles,CA 90025,USA

出　　处：《Journal of Traditional Chinese Medical Sciences》2024年第4期500-512,共13页中医科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(81590952);the“Jiebang Guashuai”Project of the Beijing University of Chinese Medicine(2022-JYB-JBZR-024).

摘　　要：Objective:To elucidate the differences in manual acupuncture effectiveness at sensitized points by investigating the mechanisms of local skin action at different sensitization points in rats with knee osteoarthritis(KOA).Methods:Forty SpragueeDawley rats were equally divided into control,model(1 mg of mono-iodoacetate into the right knee joint cavity),sham operation,manual acupuncture at right Tianjing acupoint(MAR-SJ 10),and left SJ 10 groups.Safranine-O and fast green staining were used to assess the modeling.The morphological and functional changes in mast cells(MCs)were assessed during acupoint sensitization using toluidine blue and immunofluorescence staining.The levels of serotonin,histamine,substance P(SP),and tryptase at skin acupoints and serum levels of IL-β,IL-6,and TNF-αwere detected using ELISA.Results:After 14 days of treatment,the number of MCs and their degranulation rates were statistically higher in the model group than in the control group(both P<0.001).After applying acupuncture,the levels of 5-HT,HA,and SP at skin acupoints were lower than those in the model group(all P<0.05),and tryptase level was higher(both P<0.05).Tryptase level was higher on the skin at the MAL-SJ 10 acupoint than that on the MAR-SJ 10 acupoint(P=0.004).Compared with the model group,the serum levels of IL-1β,IL-6,and TNF-αin the MAR-SJ 10 and MAL-SJ 10 groups were lower(all P<0.05).Conclusion:Acupuncture at KOA-sensitized acupoints mitigates joint injury in KOA rats and may bidi-rectionally regulate local MCs of these acupoints.This finding not only enhances the reference value of sensitizing points in clinical diagnosis and treatment,but also contributes to the understanding of the biological mechanisms underlying acupuncture intervention at sensitizing points.

关 键 词：Knee osteoarthritis ACUPUNCTURE Acupoint sensitization Mast cells SEROTONIN HISTAMINE Substance P TRYPTASE 

分 类 号：R246.9[医药卫生—针灸推拿学]