The role of brevican regulation in the antidepressant effects of electroacupuncture in a chronic stress rat model  

作　　者：Cong Gai Zhenyu Guo Kai Guo Shixin Yang Yi Zhang Huimin Zhu Feifei Kan Hongmei Sun Die Hu 

机构地区：[1]School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing,102488,China

出　　处：《Journal of Traditional Chinese Medical Sciences》2024年第4期513-521,共9页中医科学杂志（英文）

基　　金：funded by the National Natural Science Foundation of China(81803857);the Scientific Research In-depth Development Fund of Beijing University of Chinese Medicine(2022-ZXFZJJ-008).

摘　　要：Objective:To investigate the mechanism of electroacupuncture(EA)for treating depression and to explore the role of brevican in the medial prefrontal cortex(mPFC)in modulating stress susceptibility and the antidepressant effects of EA in rats.Methods:Twenty-four SpragueeDawley(SD)rats were equally divided into three groups:green fluo-rescent protein(GFP)+control,GFP+chronic unpredicted mild stress(CUMS),and short-hairpin RNA targeting on brevican(shBcan)+CUMS.Another 24 SD rats were equally divided into CUMS+GFP,CUMS+GFP+EA,and CUMS+shBcan+EA groups.Behavioral tests were conducted to assess depression-like behavior.Western blot analysis was used to evaluate the expression of brevican,aggrecan,GLuA1,and PSD95 in mPFC subregions.Results:Behavioral parameter evaluation show that rats in the shBcan+CUMS group exhibited a significantly reduced sucrose preference(P=0.0002)and increased immobility time(P=0.0011)compared to those in rats in the GFP+CUMS group.Western blotting showed that brevican expression was significantly downregulated in the PrL of the shBcan+CUMS group compared with that in the GFP+CUMS group(P=0.0192).Furthermore,compared to the CUMS+GFP+EA group,the CUMS+shBcan+EA group exhibited a significantly decreased sucrose preference(P=0.0334),increased immobility time(P=0.0465),and increased latency to food(P=0.0261).In the CUMS+shBcan+EA group,the EA-induced brevican and PSD95 overexpression was reversed,compared with that in the CUMS+GFP+EA group(P=0.0454 and P=0.0198,respectively).Conclusion:EA exerts its antidepressant effects through the modulation of brevican expression in rats.Our findings highlight the important role for brevican in stress susceptibility,which could be a potential target for treating depression.

关 键 词：DEPRESSION ELECTROACUPUNCTURE BREVICAN Perineuronal nets PLASTICITY 

分 类 号：R285.5[医药卫生—中药学]