去氢骆驼蓬碱衍生物BCN-3-5对三阴性乳腺癌恶性特征的影响  

The effect of harmine derivative BCN-3-5 on the malignant features of triple-negative breast cancer

作　　者：许芮 陈艳艳[1] 单美慧[1] 杨乐[1] 宋海涛[1] 乔雷 张楠[1] 马斌林[1] 董朝[1] Xu Rui;Chen Yanyan;Shan Meihui;Yang Le;Song Haitao;Qiao Lei;Zhang Nan;Ma Binlin;Dong Chao(Xinjiang Medical University Affiliated Cancer Hospital,Xinjiang Breast and Thyroid Tumor Clinical Medical Research Center,Urumqi 830011,China)

机构地区：[1]新疆医科大学附属肿瘤医院,新疆乳腺甲状腺肿瘤临床医学研究中心,乌鲁木齐830011

出　　处：《中华实验外科杂志》2024年第11期2510-2513,共4页Chinese Journal of Experimental Surgery

基　　金：科技创新团队(天山创新团队)项目(2022TSYC7D0017)。

摘　　要：目的:探讨去氢骆驼蓬碱衍生物BCN-3-5对三阴性乳腺癌(TNBC)细胞增殖、凋亡、侵袭及迁移的影响。方法:购买人三阴性乳腺癌细胞MDA-MB-231及MDA-MB-468各60株作为研究对象,采用简单随机分组法将MDA-MB-231及MDA-MB-468细胞株各分为5组,每组12株,分别用药物进行干预,分为空白对照组、BCN-3-5组、顺铂组、sAJM589组、10058-F4组,采用细胞活性检测试剂盒(CCK-8法)检测细胞增殖,Transwell法检测细胞迁移和侵袭,流式细胞术检测细胞凋亡。建立斑马鱼肿瘤移植模型共100尾,采用简单随机分组法分为3组,每组20尾,分为模型空白对照组、模型顺铂组、模型BCN-3-5组,通过测定肿瘤细胞荧光强度分析BCN-3-5抗乳腺癌肿瘤生长及凋亡作用。统计学结果用均值±标准差(x±s)表示,组间均数比较使用独立样本t检验、非参数检验。结果:CCK-8结果显示,BCN-3-5组存活率明显高于空白对照组[(77.001±6.261)%比(99.999±7.652)%,t=5.213,P<0.05],差异有统计学意义。Transwell法结果显示,BCN-3-5组迁移数低于空白对照组迁移数[(142.000±18.193)个比(255.000±24.000)个,t=6.499,P<0.05];BCN-3-5组侵袭数低于空白对照组迁移数[(124.333±22.368)个比(249.667±17.039)个,t=7.720,P<0.05],差异有统计学意义。流式细胞术结果显示,BCN-3-5组细胞凋亡率高于空白对照组[(10.913±1.432)%比(3.313±0.351)%,t=8.928,P<0.05],差异有统计学意义。斑马鱼移植瘤模型实验结果显示,模型BCN-3-5组抑制肿瘤生长与促进肿瘤凋亡作用强于模型空白对照组[(246826±276370)像素比(419489±47545)像素,t=9.929,P<0.05;(1689918±58288)像素比(1307668±38241)像素,t=17.34,P<0.05],模型BCN-3-5组抑制肿瘤生长与促进肿瘤凋亡作用强于模型顺铂组[(246826±27637)像素比(285347±40576)像素,t=2.481,P<0.05;(1689918±58288)像素比(1601809±93033)像素,t=2.538,P<0.05],差异有统计学意义。结论:去氢骆驼蓬碱衍生物BCN-3-5能够抑制三阴性乳腺癌恶�Objective To investigate the effects of BCN-3-5 on cell proliferation,apoptosis,migration,invasion in triple negative breast cancer(TNBC)cells.Methods Sixty MDA-MB-231 and MDA-MB-468 triple-negative breast cancer cells were procured as research objects.Both the MDA-MB-231 and MDA-MB-468 cell lines were randomly divided into 5 groups each through the simple random grouping method,12 plants per group.The cells were intervened with drugs and classified into a blank control group,a BCN-3-5 group,a cisplatin group,an SAJM589 group,and a 10058-F4 group.Cell proliferation was detected using the cell viability assay kit(CCK-8 method),cell migration and invasion were detected using the Transwell assay,and cell apoptosis was detected by flow cytometry.100 zebrafish tumor transplantation model was established and randomly divided into 3 groups with 20 individuals in each group,namely a model blank control group,a model cisplatin group,and a model BCN-3-5 group.The anti-tumor growth and apoptosis effects of BCN-3-5 on breast cancer were analyzed by measuring the fluorescence intensity of tumor cells.The statistical results were expressed as mean±standard deviation(x±s),and the comparison of group means was conducted using independent sample t-tests and non-parametric tests.ResultsThe CCK8 results showed that the survival rate of BCN-3-5 group was significantly higher than that of the blank control group[(77.001±6.261)%vs.(99.999±7.652)%,t=5.213,P<0.05],with a statistically significant difference.The results of the Transwell assay showed that the number of migrating cells in the BCN-3-5 group was lower than that in the blank control group(142.000±18.193 vs.255.000±24.000,t=6.499,P<0.05);the number of invasive cells in the BCN-3-5 group was lower than that in the blank control group(124.333±22.368 vs.249.667±17.039,t=7.720,P<0.05),with a statistically significant difference.The flow cytometry results showed that the rate of cell apoptosis in the BCN-3-5 group was higher than that in the blank control group[(10.913±1.4

关 键 词：三阴性乳腺癌 去氢骆驼蓬碱衍生物 BCN-3-5 C-MYC 

分 类 号：R737.9[医药卫生—肿瘤]