幽门螺杆菌分子检测技术研究进展  

Research progress on molecular detection technology of Helicobacter pylori

作　　者：崔玉峰 林毅军 王志民 Cui Yufeng;Lin yjun;Wang Zhimin(Shanghai BEION Pharmaceutical Technologies Co.,LTD,Shanghai 200949,China;Department of Gastroenterology,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China)

机构地区：[1]上海北昂医药科技股份有限公司,上海200949 [2]首都医科大学附属北京地坛医院消化科,北京100015

出　　处：《中华实验和临床感染病杂志（电子版）》2024年第5期257-262,共6页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：国家重点研发计划(No.2023YFC230)。

摘　　要：幽门螺杆菌(Hp)感染诊断金标准通常为组织病理、细菌培养或13C/14C尿素呼气试验。组织学检查和细菌培养为侵入性检查,技术要求高、耗时长,不适用于儿童及患有基础疾病的老年人。13C尿素呼气试验价格高,14C有放射性不推荐儿童或孕妇使用,且尿素呼气试验易受质子泵抑制剂、抗菌药物等药物干扰。分子检测在菌株分型、抗菌药物耐药筛选具有优势,而且样本来源多样,侵入性或非侵入性检测均能应用。本文对Hp分子检测技术做一综述。Histopathology,bacterial culture or 13C/14C urea breath test are usually considered as the gold standards for diagnosis of Helicobacter pylori(H.pylori)infection.Histologic examination and bacterial culture are invasive,with high technical requirements and long process.Therefore,they are not applicable to children and elderly patients with underlying diseases.13C urea breath test is expensive,14C is radioactive and not recommended for children or pregnant women,urea breath test is easily interfered by drugs such as proton pump inhibitors,antibiotics and other drugs.Molecular tests have advantages on strain typing and antibiotic resistance screening,and can use different sample types from both invasive and non-invasive approaches.This article provides a review of the molecular detection techniques for H.pylori.

关 键 词：幽门螺杆菌 分子诊断 检测 

分 类 号：R573[医药卫生—消化系统]