布莱凯特黑牛胎盘印记和营养转运基因表达及其与初生重相关性分析  

Study on the expression of placental imprint genes and nutrient transport genes and their correlation analysis with newborn weight in Shandong black cattle

作　　者：陈振鹏 刘瑞莉 张文琦 董雅娟 柏学进 CHEN Zhenpeng;IU Ruili;ZHANG Wenqi;DONG Yajuan;BAI Xuejin(College of Animal Science,Qingdao Agricultural University,Qingdao 266109,China;Innovation Center for Shandong Black Cattle Breeding Technology,Qingdao 266109,China)

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]黑牛种业技术创新中心,山东青岛266109

出　　处：《黑龙江畜牧兽医》2024年第24期35-42,136,137,共10页Heilongjiang Animal Science And veterinary Medicine

基　　金：山东省现代化农业产业技术体系牛产业创新团队项目(6682216030)。

摘　　要：为了探究胎盘印记和营养转运基因与新生牛初生重的相关性,以鉴定新生牛生长发育候选标记基因,试验选取初生重为26.5~31.5 kg和32.0~36.0 kg的新生健康布莱凯特黑牛各3头,分别记为低初生重组(LW组)和高初生重组(HW组),采集两组的胎盘组织并提取RNA,利用实时荧光定量PCR分析两组胎盘中印记基因[水通道蛋白1(aquaporin 1,AQP1)、小核核糖核蛋白多肽N(small nuclear ribonucleoprotein polypeptide N,SNRPN)、致命-3恶性脑肿瘤样蛋白1(lethal-3 malignant brain tumor-like protein 1,L3MBTL1)、pleckstring同源性样结构域家族A成员2(pleckstring homology-like domain family A member 2,PHLDA2)、胰岛素样生长因子2(insulin like growth factor 2,IGF2)、生长因子受体结合蛋白10(growth factor receptor bound protein 10,GRB10)]和营养转运基因[溶质载体家族38成员10(solute carrier family 38 member 10,SLC38A10)、葡萄糖转运蛋白家族2成员1(solute carrier family 2 member 1,SLC2A1)、葡萄糖转运家族2蛋白2(solute carrier family 2 member 2,SLC2A2)、脂肪酸转运蛋白家族27成员1(solute carrier family 27 member 1,SLC27A1,也称FATP1)、质子依赖性胃肠肽转运蛋白1(proton-dependent gastrointestinal peptide transporter 1,PEPT1,也称SLC15A1)、脂肪酸转运蛋白家族27成员4(solute carrier family 27 member 4,SLC27A4,也称FATP4)、钠依赖型葡萄糖转运载体1(Na+/glucose cotransporter 1,SGLT1,也称SLC5A1)、白细胞分化抗原36(cluster of differentiation 36,CD36)]的mRNA表达水平差异,并分析上述基因表达量与初生重间的相关性,最后对上述基因进行生物信息学分析。结果表明:LW组平均初生重[(30.00±0.87)kg]和HW组[(34.17±1.17)kg]差异显著(P<0.05);胎盘印记基因AQP1、SNRPN、L3MBTL1、PHLDA2、IGF2、GRB10在HW组和LW组胎盘中均有表达,其中HW组AQP1、SNRPN、L3MBTL1、PHLDA2基因mRNA相对表达量显著高于LW组(P<0.05),IGF2、GRB10基因显著低于LW组(P<0.05)。胎盘营养转运基因SLC38A10In order to investigate the correlation between placental imprint genes and nutrient transport genes and newborn weight of cattle to identify candidate marker genes for newborn cattle growth and development,in the experiment,each three newborn healthy Shandong black cattle with the newborn weight of 26.5-31.5 kg and 32.0-36.0 kg were selected,and were recorded as low newborn group(LW group)and high newborn group(HW group),respectively.Placental tissues were collected from two groups of cattle and RNA was extracted.Real-time fluorescence quantitative PCR was used to analyze the differences in expression levels of the imprint genes(aquaporin 1[AQP1],small nuclear ribonucleoprotein polypeptide N(SNRPN),lethal-3 malignant brain tumor-like protein 1[L3MBTL1],pleckstring homology-like domain family A member 2[PHLDA2],insulin like growth factor 2[IGF2],growth factor receptor bound protein 10[GRB10])and of the nutrient transport genes(solute carrier family 38 member 10[SLC38A10],solute carrier family 2 member 1[SLC2A1],solute carrier family 2 member 2[SLC2A2],solute carrier family 27 member 1[SLC27A1 or FATP1],proton-dependent gastrointestinal peptide transporter 1[PEPT1 or SLC15A1],solute carrier family 27 member 4[SLC27A4 or FATP4],Na~+/glucose cotransporter 1[SGLT1 or SLC5A1],and cluster of differentiation 36[CD36]).And the correlation between the expression of these genes and newborn weight was analyzed,and these genes were analyzed bioinformatically finally.The results showed that there was a significant difference in the average newborn weight between the LW group([30.00±0.87]kg)and the HW group([43.17±1.17]kg)of Shandong black cattle.The placental imprint genes AQP1,SNRPN,L3MBTL1,PHLDA2,IGF2 and GRB10 were expressed in the placentas of both the HW and LW groups;the relative mRNA expression of the genes AQP1,SNRPN,L3MBTL1 and PHLDA2 in the HW group was significantly higher than those in the LW group(P<0.05),and the relative mRNA expression of IGF2 and GRB10 genes was significantly lower than those in the LW group

关 键 词：布莱凯特黑牛 胎盘 印记基因 营养转运基因 新生牛 初生重 

分 类 号：S823.2[农业科学—畜牧学]