一例干扰素受体缺失小鼠自发未成熟型睾丸畸胎瘤诊断  

Preliminary Diagnosis and Characterization of a Spontaneous Immature Testicular Teratoma in an Interferon Receptor-Deficient Mouse Model

作　　者：赵赫 张帆[1] 肖宇宙[1] 安学芳[1] 张涛[1] 李丽 ZHAO He;ZHANG Fan;XIAO Yuzhou;AN Xuefang;ZHANG Tao;LI Li(Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430070,China)

机构地区：[1]中国科学院武汉病毒研究所,武汉430070

出　　处：《实验动物与比较医学》2024年第6期691-694,共4页Laboratory Animal and Comparative Medicine

基　　金：国家重点研发计划项目“基于新靶点的重大传染病人源化动物模型研发”(2021YFF0702000);湖北省科技厅项目“实验动物生物安全标准化建设”(2022DFE023)。

摘　　要：目的观察干扰素受体缺失小鼠(AG129)睾丸自发性肿瘤,为该品系小鼠的应用研究提供依据。方法AG129小鼠种群饲养于SPF屏障环境中,正常饲养繁殖,在离乳的3周龄小鼠中发现其中一只雄性小鼠睾丸异常肿大,同窝内其他小鼠无相似情况,对该例小鼠自发的睾丸肿瘤进行持续观察,于9周龄时对小鼠安乐死,并解剖,观察肿瘤组织大体形态并取材,肿瘤组织经石蜡切片后进行HE染色。结果小鼠经过持续数周观察发现,肿瘤组织随小鼠日龄增长逐渐增大;解剖后肿瘤组织被包囊包裹,表面光滑,切开包囊后,肿瘤内组织形态不规则,部分质地坚硬,部分柔软;HE染色结果显示,肿瘤组织由多胚层组织发育而成,内部有神经组织、骨组织、肌肉组织和脂肪组织等,形态结构不规则。结论结合肿瘤发生发展,大体形态结构,病理切片HE染色结果,初步诊断该例小鼠睾丸肿块为小鼠未成熟型畸胎瘤。Objective To observe the spontaneous testicular tumors in interferon receptor-deficient mice(AG129)and provide a basis for further research using this mouse strain.Methods The AG129 mouse population was bred in an SPF barrier environment and reproduced normally.Among the 3-week-old weaned mice,we found that one male mouse had an abnormally enlarged testis,while none of the other mice in the same litter exhibited a similar condition.The spontaneous testicular tumor in this mouse was continuously monitored.The mouse was euthanized and dissected at 9 weeks of age.The gross morphology of the tumor was observed,and the tissue was then embedded in paraffin and sectioned for hematoxylin and eosin(HE)staining.Results After several weeks of observation,the tumor tissue gradually increased in size as the mice aged.Upon dissection,the tumor tissue was encapsulated in a smooth capsule.After opening the capsule,the tumor tissue was irregular in shape,with some areas being hard and others soft.The results of HE staining showed that the tumor tissue developed from tissues derived from multiple germ layers,with nerve tissue,bone tissue,muscle tissue,and adipose tissue inside,exhibiting an irregular morphological structure.Conclusion Based on the tumor development,gross morphological structure,and the HE staining results of the pathological sections,the tumor in this mouse was preliminarily diagnosed as an immature teratoma.

关 键 词：干扰素受体 小鼠 睾丸 未成熟型畸胎瘤 

分 类 号：R737[医药卫生—肿瘤] Q95-33[医药卫生—临床医学]