固相萃取-高效液相色谱法测定红茶与红糖中紫胶红色素  

Determination of Laccaic Acids in Black Tea and Brown Sugar by Solid Phase Extraction-High Performance Liquid Chromatography

作　　者：孟鹏 MENG Peng(National Quality Supervision and Testing Center for Processed Food(FuZhou),Fujian Inspection and Research Institute for Product Quality,Fuzhou 350002)

机构地区：[1]国家加工食品质量检验检测中心(福州),福建省产品质量检验研究院,福建福州350002

出　　处：《分析科学学报》2024年第6期703-708,共6页Journal of Analytical Science

基　　金：福建省科学技术厅科技项目(2023N0030);福建省市场监督管理局科技项目(FJMS2022005);福建省产品质量检验研究院院级储备项目(KY202204A)。

摘　　要：建立了红茶和红糖中紫胶红色素的固相萃取-高效液相色谱法。采用纯水进行超声辅助萃取样品,经聚酰胺(PA)固相萃取柱富集后在XB-C_(18)(50 mm×4.6 mm,3.0μm)色谱柱上分离,用甲醇-0.02 mol/L乙酸铵水溶液(含0.009%的磷酸)作为流动相进行梯度洗脱,光电二极管阵列检测器进行检测。结果表明,紫胶红色素各组分在0.023~5.86 mg/L范围内具有良好的线性关系,相关系数均>0.999,定量限为0.025~0.047 mg/kg。方法的加标回收率为96.2%~104.6%,相对标准偏差为0.6%~4.0%。该方法在普通液相色谱仪器上7 min即可实现紫胶红色素各组分的分离,灵敏度高,重现性好,抗干扰能力强,适用于红茶和红糖中天然色素紫胶红的定量检测。A new method was established for the determination of laccaic acids in black tea and brown sugar by solid phase extraction-high performance liquid chromatography(SPE-HPLC).The samples were ultrasonic extracted by water,then cleaned up by a polyamide(PA)SPE column.The chromatographic separation was achieved on an XB-C 18 column(50×4.6 mm,3.0μm)by linear gradient elution with methanol/ammonium acetate solution(containing 0.009% phosphoric acid)as the mobile phase.The results showed that there was a good linear correlation(r>0.999)between the peak area and concentration of laccaic acids in the range of 0.023-5.86 mg/L.The limits of quantification(LOQs)of laccaic acids were 0.025-0.047 mg/kg.The spiked recoveries for the samples and relative standard deviations(RSDs)were 96.2%-104.6% and 0.6%-4.0%,respectively.The separation of laccaid acids could be achieved within 7 minutes on common liquid chromatography instrument.It was sensitive and repeatable for the quantitative determination of laccaic acids in black tea and brown sugar with good anti-interference ability.

关 键 词：红茶 红糖 紫胶红 固相萃取 高效液相色谱 

分 类 号：O657.72[理学—分析化学]