Esperase蛋白酶对柞蚕茧的脱胶工艺及蚕丝性能  

Degumming process and silk properties of tussah cocoon using Esperase

作　　者：冉辉 张楠[1] 汪媛[1] 魏艳红 纪俊玲[1] RAN Hui;ZHANG Nan;WANG Yuan;WEI Yanhong;JI Junling(Changzhou University,Changzhou 213159,Jiangsu,China;Jiangsu Gem Advanced Fiber Materials Research Institute Co.,Ltd.,Nantong 226000,Jiangsu,China)

机构地区：[1]常州大学,江苏常州213159 [2]江苏集萃先进纤维材料研究所有限公司,江苏南通226000

出　　处：《上海纺织科技》2024年第12期30-34,39,共6页Shanghai Textile Science & Technology

基　　金：常州大学科研启动经费项目(ZMF23020026);纺织行业医疗健康用蚕丝制品重点实验室(SDHY2233);国家自然科学基金(52303004)。

摘　　要：以活性高、底物广谱的Esperase蛋白酶作为生物脱胶剂,分别探究酶用量和酶处理时间对柞蚕茧脱胶率的影响,并优化脱胶条件;利用SDS-PAGE、扫描电子显微镜(SEM)和纤维强力仪对脱胶液和脱胶丝素的理化性质进行研究,并与碳酸钠脱胶法进行对比。结果表明:最佳脱胶工艺条件为每克柞蚕茧的酶用量100μL,温度50℃,处理2 h。在最佳脱胶工艺条件下,脱胶率为12.83%,丝胶蛋白被水解成分子量小于10000 Da的小分子而被去除,丝素表面光滑平整,蚕丝的断裂强力略微下降,韧性变好。与碱法脱胶相比,Esperase蛋白酶具有更均匀的脱胶效果。Esperase protease with high activity and a broad substrate spectrum is used as a biology degumming agent,the effects of enzyme dosage and enzyme treatment time on the degumming rate of tussah cocoon are investigated,and the enzymatic degumming process is optimized.The physicochemical properties of degumming solution and degumming silk fibroin are characterized using SDS-PAGE,scanning electron microscopy(SEM)and fiber strength meter,and compared with those of sodium carbonate degumming method.The results show that the optimum degumming conditions are 100μL enzyme dosage per gram of tussah cocoon,treating at 50℃ for 2 h.Under the optimal degumming conditions,the degumming rate is 12.83%,sericin is hydrolyzed into small molecules with a molecular weight less than 10000 Da;the surface of silk fibroin is smooth and flat,the breaking strength of degummed silk slightly decreases while the toughness improves.Compared with alkali degumming,enzymatic degumming method with esperase has a more uniform degumming effect.

关 键 词：柞蚕茧 脱胶 Esperase蛋白酶 抗氧化性 拉伸强力 

分 类 号：TS143.3[轻工技术与工程—纺织材料与纺织品设计]