胰腺癌细胞源性外泌体调控神经源细胞功能的机制  

Mechanism of Pancreatic Cancer Cell Derived Exosomes Regulating the Function of Neurogenic Cells

作　　者：黄文康 吴思慧 徐岷[2] HUANG Wenkang;WU Sihui;XU Min(Department of Hepatobiliary,Pancreatico-Splenic Surgery,Affiliated Hospital of Jiangsu University,Jiangsu 212000,China)

机构地区：[1]江苏大学附属医院肝胆胰脾外科,镇江212000 [2]江苏大学附属医院消化科,镇江212000

出　　处：《医学研究杂志》2024年第12期62-66,共5页Journal of Medical Research

基　　金：国家自然科学基金资助项目(82072754);江苏省重点研发计划项目(BE2018689)。

摘　　要：目的探讨胰腺癌细胞源性外泌体调控神经源细胞功能的机制。方法加入TrkA抑制剂GW441756后,采用Western blot法检测胰腺癌细胞源性外泌体对神经源细胞中神经生长因子/原肌球蛋白受体激酶A(nerve growth factor/tropomyosin receptor kinase A,NGF/TrkA)表达水平的影响;通过CCK-8实验及克隆形成实验探讨胰腺癌细胞源性外泌体及NGF/TrkA对神经源细胞增殖能力的影响,通过Transwell实验探讨胰腺癌细胞源性外泌体及NGF/TrkA对神经源细胞迁移能力的影响。结果胰腺癌细胞源性外泌体可以上调神经源细胞中的NGF/TrkA表达水平,其作用可以被TrkA抑制剂GW441756所抑制;加入TrkA抑制剂GW441756后,神经源细胞的增殖和迁移能力受到抑制,而与外泌体共培养后,其抑制作用可以被逆转。结论胰腺癌细胞源性外泌体通过上调神经源细胞中NGF/TrkA水平从而影响神经源细胞的功能。Objective To investigate the mechanism of regulation of neurogenic cell function by pancreatic cancer cell-derived exosomes.Methods After TrkA inhibitor GW441756 was added,the effect of pancreatic cancer cell-derived exosomes on nerve growth factor/tropomyosin receptor kinase A(NGF/TrkA)expression level of neurogenic cells was detected by Western blot.The effects of pancreatic cancer cell-derived exosomes and NGF/TrkA on the proliferation capacity of neurogenic cells were investigated by CCK-8 assay and clonal formation assay,and the effects of pancreatic cancer cell-derived exosomes and NGF/TrkA on the migration capacity of neurogenic cells were investigated by Transwell assay.Results Pancreatic cancer cell-derived exosomes could up-regulate the expression of NGF/TrkA in neurogenic cells,and its effect can be inhibited by TrkA inhibitor GW441756.After the addition of TrkA inhibitor GW441756,the proliferation and migration ability of neurogenic cells were inhibited,and the inhibition effect could be reversed after co-culture with exosomes.Conclusion Pancreatic cancer cell-derived exosomes affect the function of neurogenic cells by up-regulating NGF/TrkA levels in neurogenic cells.

关 键 词：胰腺癌 外泌体 NGF/TrkA 增殖 迁移 

分 类 号：R576[医药卫生—消化系统]