巴西产酒神菊正己烷部位化学成分及其体外抗肝损伤活性研究  

Chemical constituents from the n-hexane fraction of Baccharis trimera from Brazil and their in vitro anti-liver injury activities

作　　者：杨雄 刘宏栋[1] 蒋涛 闵建新[1] 李斌[1] YANG Xiong;LIU Hong-dong;JIANG Tao;MIN Jian-xin;LI Bin(Workstation for Academician,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学院士工作站,江西南昌330004

出　　处：《中成药》2024年第12期4042-4048,共7页Chinese Traditional Patent Medicine

基　　金：中央本级重大增减支项目(2060302-2101-10)。

摘　　要：目的 研究巴西产酒神菊正己烷部位的化学成分及其体外抗肝损伤作用。方法 采用GC-MS和LC-MS鉴定化学成分其及入血成分。采用乙醇诱导的LO2细胞评价其抗肝损伤作用,并检测细胞存活率和细胞ROS、 ALT、 AST、GSH、 GST水平。结果 从正己烷部位中分离鉴定出喇叭茶醇(8.55%)、绿花白千层醇(6.83%)、桉油烯醇(4.26%)等54个化合物,含药血清中鉴定出石竹素、(-)-α-荜澄茄油烯等7个原型成分。与模型组组比较,含药血清组的细胞存活率明显升高(P<0.01),ALT、 AST、 ROS水平显著下降(P<0.01),GST、 GSH水平显著升高(P<0.05)。结论 巴西产酒神菊正己烷部位具有体外具有一定的抗氧化活性和保肝作用,发挥抗氧化活性作用的可能是倍半萜类物质。AIM To study the chemical constituents from then-hexane fraction ofBaccharis trimera(Less.)DC from Brazil and theirin vitroanti-liver injury activities.METHODS The chemical constituents and blood components were identified by GC-MS and LC-MS.The ethanol-induced LO2 cells were used to evaluate their antiliver injury effects,and the cell survival rate and the levels of ROS,ALT,AST,GSH and GST were detected.RESULTS A total of 54 compounds were isolated and identified fromn-hexane fraction,including (-)-palustrol(8.55%),viridiflorol(6.83%),spathulenol(4.26%),and 7 prototype components such as caryophyllin and(-)-α-cubebene were identified from the drug-containing serum.Compared with the model group,the cell viability rate of drug-containing serum group was significantly increased(P<0.01),and the levels of ALT,AST and ROS were significantly decreased(P<0.01),and the levels of GST and GSH were significantly increased(P<0.05).CONCLUSION Then-hexane fraction from theBaccharis trimerahas a certainin vitroantioxidant activity and hepatoprotective effects.It's possible that sesquiterpenoids play a role in antioxidant activity.

关 键 词：酒神菊 化学成分 GC-MS 含药血清 LO2细胞 抗氧化活性 抗肝损伤 

分 类 号：R284.1[医药卫生—中药学]