Coal Worker’s Pneumoconiosis-Targeted Lipidomics Reveals Aberrant Phospholipid Metabolism for Early-Stage Diagnosis  

作　　者：Yuzhen Feng Jing Dai Junyun Wang Fangda Peng Wenrong Wang Xiao Yu Xiaomei Kong Qingjun Qian Qicai Liu Huanqiang Wang Chunguang Ding Yiwei Shi 

机构地区：[1]Guangdong Pharmaceutical University,Guangzhou City,Guangdong Province,China [2]National Center for Occupational Safety and Health,NHC,Beijing,China [3]NHC Key Laboratory for Engineering Control of Dust Hazard,Beijing,China [4]National Institute for Occupational Health and Poison Control,Chinese Center for Disease Control and Prevention,Beijing,China [5]NHC Key Laboratory of Pneumoconiosis,MOE Key Laboratory of Coal Environmental Pathogenicity and Prevention,Shanxi Key Laboratory of Respiratory Diseases,The First Hospital of Shanxi Medical University,Taiyuan City,Shanxi Province,China.

出　　处：《China CDC weekly》2024年第52期1410-1416,I0004,I0005,共9页中国疾病预防控制中心周报（英文）

摘　　要：Introduction:Pneumoconiosis is the most prevalent occupational disease in China,with coal worker pneumoconiosis(CWP)demonstrating the highest incidence.Studies have indicated that phospholipids may be associated with CWP.Methods:In this study,serum was obtained from 62 patients with pneumoconiosis,105 coal dustexposed workers,and 50 healthy individuals and analyzed via targeted lipidomics using ultraperformance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).After initially identifying phospholipids with significant differences through univariate and multivariate statistical analyses,receiver operating characteristic(ROC)analysis was performed.The differential phospholipids identified in patient samples were then integrated to assess their diagnostic potential for CWP using a support vector machine(SVM).Results:Compared with healthy subjects,the levels of Lyso-PS(18:0)were decreased,while PC(16:0),PC(18:0),PC(16:0/18:1),PI(16:0/18:1),PS(18:1),PG(16:0),and PG(18:0/18:1)were significantly increased in the pneumoconiosis group,with an area under the curve(AUC)>0.7.Moreover,compared with the dust-exposed group,Lyso-PC(16:0),PC(16:0),PC(16:0/18:1),PI(16:0/18:1),and PG(16:0)were significantly elevated in the pneumoconiosis group,with an AUC>0.7.The diagnostic model,including PC(16:0),PC(16:0/18:1),PI(16:0/18:1),and PG(16:0),demonstrated excellent performance with an AUC of 0.956.Discussion:The serum phospholipid profiles of patients with pneumoconiosis differed significantly from those of controls,including differences in PC,Lyso-PC,PI,PS,Lyso-PS,and PG.Among these,a diagnostic model incorporating PC(16:0),PC(16:0/18:1),PI(16:0/18:1),and PG(16:0)demonstrated superior screening efficiency.

关 键 词：LIPID ELEVATED initially 

分 类 号：R13[医药卫生—劳动卫生] R589[医药卫生—公共卫生与预防医学]