桑叶多糖对黄曲霉毒素B1诱导肝损伤的保护作用  

Protective effect of mulberry leaf polysaccharides on AFB1-induced liver injury

作　　者：邝甜甜 孙筱梦 史心茹 张苑 谢宗固 司红彬[1] KUANG Tiantian;SUN Xiaomeng;SHI Xinru;ZHANG Yuan;XIE Zonggu;SI Hongbin(College of Animal Science and Technology,Guangxi University/Forage Workstation of Guangxi Zhuang Autonomous Region,Nanning 530000,China)

机构地区：[1]广西大学动物科学技术学院/广西壮族自治区牧草工作站,广西南宁530000

出　　处：《畜牧与兽医》2025年第1期119-125,共7页Animal Husbandry & Veterinary Medicine

基　　金：中央引导地方专项[桂科计字(2023)55号];国家自然基金联合基金项目(U22A20523);广西大学自科创新倍增计划项目(2024BZPT020);广西大学“大学生创新创业训练计划”项目(S202210593208);南宁市重点研发计划项目(20232051)。

摘　　要：旨在探究桑叶多糖(MLP)对黄曲霉毒素B1(AFB1)诱导小鼠肝损伤的保护作用及其潜在机制。将50只昆明小鼠随机分为空白组、模型组、阳性对照组、MLP低剂量组和MLP高剂量组,每组10只。模型组小鼠灌胃给予生理盐水(每10 g体重0.1 mL),阳性对照组灌胃给予水飞蓟素(每千克体重100 mg),MLP低剂量组和MLP高剂量组灌胃给予桑叶多糖(每千克体重200、400 mg),2 h后,模型组、阳性对照组、MLP低剂量组和MLP高剂量组采用灌胃方式给予AFB1(每千克体重0.75 mg),空白组再次灌胃生理盐水(每10 g体重0.1 mL)。连续灌胃14 d,麻醉后经眼眶采血,处死小鼠。检测小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)的活性;检测血清中超氧化物歧化酶(SOD)、谷胱甘肽S转移酶(GST)、小鼠细胞色素P4502E1(CYP2E1)的活性以及还原型谷胱甘肽(GSH)的含量,评价氧化应激水平;制作肝组织切片,采用苏木精-伊红法(HE)观察肝脏的组织变化;荧光定量PCR法检测肝匀浆Keap1、Nrf2、SOD1 mRNA的表达水平。结果:与空白组相比,模型组ALT、AST、ALP的活性显著增高(P<0.05),肝细胞排列杂乱无序,细胞核严重破碎,发生严重的空泡变性,GST、SOD活性显著降低(P<0.05),GSH含量显著降低(P<0.05),血清中CYP2E1活性显著升高(P<0.05),Keap1、Nrf2、SOD1的基因表达显著降低(P<0.05);与模型组相比,MLP低剂量组和MLP高剂量组能降低血清中ALT、AST、ALP、CYP2E1活性(P<0.05),提高GST、SOD活性以及GSH含量(P<0.05),改善肝脏病变,并能上调Nrf2、SOD1的基因表达量。研究表明,MLP对AFB1诱导的肝损伤能够起到预保护作用,其作用机制可能与提高肝脏的抗氧化能力和调控Keap1/Nrf2信号通路有关。The aim of this study was to explore the protective effect and potential mechanism of mulberry leaf polysaccharides(MLP)on af-latoxin B1(AFB1)induced liver injury in mice.50 Kunming mice were obtained and randomly divided into a blank group,a model group,a positive control group,a low-dose MLP group,and a high-dose MLP group,with 10 mice in each group.The model group was given physio-logical saline(01 mL per 10 g of body weight)by gavage,the positive control group was given silymarin(100 mg/kg)by gavage,and the low-dose and high-dose MLP groups were given mulberry leaf polysaccharides(200,and 400 mg/kg,respectively)by gavage.Two hours later,the model group,the positive control group,the low-dose MLP group,and the high-dose MLP group were given AFB1(075 mg/kg)by gavage.The blank group was given two doses of physiological saline by gavage(01 mL per 10 g of body weight).After continu-ous gastric lavage for 14 days,blood samples were collected from the eye socket of the mice after anesthesia,and the mice were euthanized.Then,the activities of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),and alkaline phosphatase(ALP)in the mice were detected.The activities of superoxide dismutase(SOD),glutathione S-transferase(GST),mouse cytochrome CYP4502E1(CYP2E1),and the content of reduced glutathione(GSH)in their serum were also detected in order to evaluate the oxidative stress levels in the rodents.Next,the liver tissue slices were made and the tissue changes in the liver of the animals were observed using the hematoxylin eosin(HE)method.Finally,the QPCR method was used to detect the expression levels of Keap1,Nrf2,and SOD1 mRNA in their liver ho-mogenate.The results showed that,compared with the blank group,the activities of ALT,AST,and ALP in the model group were signifi-cantly increased(P<005).The liver cells were arranged in a disordered manner,the nucleus was severely fragmented,and severe vacuolar degeneration occurred.The activities of GST and SOD were significantly reduced(P<005),the content of GSH

关 键 词：桑叶多糖 黄曲霉毒素B1 抗氧化 保肝 

分 类 号：S854[农业科学—临床兽医学]