黄瓜-酸黄瓜异附加系鉴定、果实长度变异及其分子机制  

Identification,fruit length variation and molecular mechanism of Cucumis sativus-C.hystrix alien addition lines

作　　者：刘紫薇 冯贤波 赵勤政 夏磊 娄群峰[1] LIU Ziwei;FENG Xianbo;ZHAO Qinzheng;XIA Lei;LOU Qunfeng(College of Horticulture/National Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学园艺学院/作物遗传与种质创新利用全国重点实验室,江苏南京210095

出　　处：《南京农业大学学报》2025年第1期108-120,共13页Journal of Nanjing Agricultural University

基　　金：国家重点研发计划项目(2021YFD1200200);江苏省种业振兴“揭榜挂帅”项目(JBGS〔2021〕070);江苏省自然科学基金青年基金项目(BK20221007)。

摘　　要：[目的]本文旨在通过分析不同黄瓜-酸黄瓜异附加系的果实差异,探究附加系中导入酸黄瓜6号染色体引起黄瓜果实变短的分子机制,为黄瓜近缘野生种优异性状挖掘和利用提供理论依据。[方法]采用分子标记和Oligo-FISH鉴定黄瓜-酸黄瓜异附加系中的外源染色体,对栽培黄瓜‘北京截头'(CC3)和不同黄瓜-酸黄瓜异附加系的果实性状进行统计,开展石蜡切片和转录组测序分析,并利用实时荧光定量PCR技术(RT-qPCR)验证转录组测序结果。[结果]共鉴定出7种异附加系材料:附加酸黄瓜6号染色体CC-H06、8号染色体CC-H08、9号染色体CC-H09及10号染色体CC-H10的单体附加系;附加酸黄瓜6和10号染色体CC-H06+H10的双单体附加系;附加酸黄瓜6、7和11号染色体CC-H06+H07+H11以及附加酸黄瓜6、7、10、11和12号染色体CC-H06+H07+H10+H11+H12的多单体附加系。表型统计发现,CC-H08、CC-H09和CC-H10的子房和果实长度均与CC3相似,而CC-H06较CC3明显变短,表明酸黄瓜6号染色体导入会引起CC3果实变短。石蜡切片结果显示,CC-H06的果实细胞相对于CC3较大,但细胞数目却明显减少。转录组分析表明,CC-H06中分别有695和2 770个酸黄瓜及CC3来源的差异表达基因,主要富集在植物激素信号转导、玉米素生物合成、二萜生物合成、色氨酸代谢等激素合成通路。RT-qPCR 验证分析与转录组测定结果一致,说明结果真实可靠。[结论]酸黄瓜6号染色体导入会引起CC3果实变短和果实细胞数量减少,CC-H06的果实变短与生长素、细胞分裂素和赤霉素等植物激素共同调控有关。[Objectives]The paper aimed to explore the molecular mechanism of cucumber fruit shortening caused by the introduction of Cucumis hystrix chromosome 6 in alien addition lines(AAL)by analyzing the fruit differences of different C.sativus-C.hystrix AAL,so as to provide theoretical basis for excellent character mining and utilization of cucumber related wild species. [Methods]Molecular markers and Oligo-FISH were used to identify the alien chromosomes in C.sativus-C.hystrix AAL. The fruit traits of cultivated cucumber ‘Beijingjietou'(CC3)and different C.sativus-C.hystrix AAL were statistically analyzed,paraffin sections and transcriptome sequencing were carried out,and the results of transcriptome sequencing were verified by real-time fluorescence quantitative PCR(RT-qPCR). [Results]A total of 7 different AAL were identified,including monosomic alien additive lines with the C.hystrix chromosome 6(CC-H06),chromosome 8(CC-H08),chromosome 9(CC-H09)and chromosome 10(CC-H10);double monosomic alien additive lines with the C.hystrix chromosomes 6 and 10(CC-H06+H10);and multiple monosomic alien additional lines with the C.hystrix chromosomes 6,7 and 11(CC-H06+H07+H11)and the C.hystrix chromosomes 6,7,10,11,and 12(CC-H06+H07+H10+H11+H12),respectively. Phenotypic investigation showed that the ovary and fruit length of AAL CC-H08,CC-H09 and CC-H10 were all similar to that of CC3,while CC-H06 was significantly shorter than that of CC3,indicating that the introduction of C.hystrix chromosome 6 would lead to the fruit shortening of CC3. The results of paraffin section showed that the fruit cells of CC-H06 were larger than those of CC3,but the number of cells was significantly reduced. Transcriptomic analysis showed that there were 695 and 2 770 differentially expressed genes derived from C.hystrix and CC3 in CC-H06,respectively,which were mainly concentrated in plant hormone signal transduction,zeatin biosynthesis,diterpenoid biosynthesis,tryptophan metabolism and other hormone synthesis pathways. The results of RT-qPCR verific

关 键 词：黄瓜 酸黄瓜 异附加系(ALL) 果实 转录组 

分 类 号：S642.2[农业科学—蔬菜学]