CGF复合Bio-Oss骨粉对牙槽骨缺损大鼠成骨细胞凋亡、牙槽骨微观形态及BMP2/4的影响  

Effects of CGF combined with Bio-Oss bone powder on osteoblast apoptosis,alveolar bone morphology and BMP2/4 in rats with alveolar bone defect

作　　者：周辰 李哲 尹婷 季佳 ZHOU Chen;LI Zhe;YIN Ting;JI Jia(Heilongjiang Qiqihar Jianhua Hospital Co.,LTD.,Heilongjiang Qiqihar 161000,China;The Third Affiliated Hospital of Qiqihar Medical College,Heilongjiang Qiqihar 161000,China.)

机构地区：[1]齐齐哈尔建华医院有限责任公司,黑龙江齐齐哈尔161000 [2]齐齐哈尔医学院附属第三医院,黑龙江齐齐哈尔161000

出　　处：《临床口腔医学杂志》2024年第12期716-721,共6页Journal of Clinical Stomatology

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(编号:2020-KYYWF-0015);齐齐哈尔市科技计划创新项目(编号:GSFGG-2021317)。

摘　　要：目的:探究浓缩生长因子(concentrated growth factors, CGF)联合Bio-Oss骨粉对牙槽骨缺损大鼠成骨细胞凋亡、牙槽骨微观形态及骨形态发生蛋白(bone morphogenetic protein, BMP)2/4的影响。方法:选取50只SPF级SD雄性大鼠,随机分为正常(NO)组,模型(MO)组,CGF(CF)组,Bio-Oss骨粉(BS)组,CGF联合Bio-Oss骨粉(CB)组,每组10只,MO组、CF组、BS组以及CB组进行牙槽骨缺损建模。建模成功后,CF组给予CGF治疗,BS组进行Bio-Oss骨粉治疗,CB组给予CGF联合Bio-Oss骨粉,NO组、MO组不予治疗,取上颌骨组织进行HE染色及Micro-CT检测,TUNEL法检测成骨细胞凋亡,免疫印记法检测BMP2/4相关蛋白表达。结果:NO组上颌骨表面光滑无缺损,MO组上颌骨可见明显缺损,新生骨仅有少量再生,与MO组比较,CF组、BS组、CB组上颌骨缺损明显减轻,可见新生骨逐渐覆盖缺损区,其中CB组修复效果最佳。与NO组相比,MO组骨小梁数量、骨小梁体积降低,骨小梁分离度升高(P<0.05);与MO组相比,CF组、BS组、CB组骨小梁数量、骨小梁体积升高,骨小梁分离度降低(P<0.05),且BS组、CF组比较无明显差异(P>0.05),CB组比BS组变化明显(P<0.05)。NO组上颌骨组织结构正常,表面光滑,未见明显骨吸收及新骨生成,MO组上颌骨组织纤维排列不规则,骨吸收明显,可见大量胶原纤维增生及少量新骨生成;与MO组比较,CF组、BS组、CB组骨吸收明显减少,新骨生成明显增多;与NO组比较,MO组成骨细胞凋亡率升高(P<0.05);与MO组比较,CF组、BS组、CB组成骨细胞凋亡率降低(P<0.05),且BS组、CF组比较无明显差异(P>0.05),CB组比BS组降低明显(P<0.05)。与NO组相比,MO组上颌骨组织BMP2、BMP4蛋白表达降低(P<0.05);与MO组相比,CF组、BS组、CB组上颌骨组织BMP2、BMP4蛋白表达升高(P<0.05),且BS组、CF组比较无明显差异(P>0.05),CB组比BS组升高明显(P<0.05)。结论:CGF联合Bio-Oss骨粉对牙槽骨缺损大鼠具有显著疗效,可有效改善成骨细胞�Objective:To investigate the effects of concentrated growth factors(CGF)combined with Bio-Oss bone powder on osteoblast apoptosis,alveolar bone micromorphology and BMP2/4 in rats with alveolar bone defect.Methods:Fifty SPF SD male rats were randomly divided into normal(NO)group,model(MO)group,CGF(CF)group,Bio-Oss bone powder(BS)group and CGF combined Bio-Oss bone powder(CB)group,with 10 rats in each group.Alveolar bone defect modeling was performed in MO,CF,BS and CB groups.After successful modeling,CGF treatment was given to group CF,Bio-Oss bone powder treatment was given to group BS,CGF combined with Bio-Oss bone powder treatment was given to group CB,and no treatment was given to group NO and group MO.Maxillary bone tissue was taken for HE staining and Micro-CT detection,and osteoblast apoptosis was detected by TUNEL method.The expression of BMP2/4-related proteins was detected by immunoimprinting.Results:The maxillary surface of the NO group was smooth without defects,while the maxillary surface of the MO group was obviously defective,and the new bone only regenerated a little.Compared with the MO group,the maxillary defects of the CF group,BS group and CB group were significantly alleviated,and the new bone gradually covered the defective area,and the CB group had the best repair effect. Compared with NO group,the number and volume of trabeculae in MO groupwere decreased,and the separation degree of trabeculae was increased(P<0. 05). Compared with MO group,the number andvolume of trabeculae in CF,BS and CB groups were increased(P<0. 05),and the separation degree of trabeculae was decreased(P<0. 05). There was no significant difference between BS group and CF group(P>0. 05),and CB group had significantchange compared with BS group(P<0. 05). Maxillary bone tissue structure in group NO was normal,the surface wassmooth,and no obvious bone resorption and new bone formation were observed. Maxillary bone tissue fiber arrangement ingroup MO was irregular,obvious bone resorption was observed,and a large amount of co

关 键 词：浓缩生长因子(CGF) BIO-OSS骨粉 牙槽骨缺损 成骨细胞凋亡 牙槽骨微观形态 骨形态发生蛋白(BMP2/4) 

分 类 号：R782.1[医药卫生—口腔医学]