7,8-DHF对糖尿病大鼠视网膜的保护作用及其机制  

Protective effects of 7,8-DHF on the retina of diabetic rats and its mechanism

作　　者：杨爱萍 郑新宝 陈春峰 陈佳玉 夏静[1] 李明芳 吴陆赟 赵永旺 YANG Aiping;ZHENG Xinbao;CHEN Chunfeng;CHEN Jiayu;XIA Jing;LI Mingfang;WU Luyun;ZHAO Yongwang(Songjiang Hospital Affiliated To Shanghai Jiao Tong University School of Medicine,Shanghai 201600,China)

机构地区：[1]上海交通大学医学院附属松江医院,上海市201600

出　　处：《眼科新进展》2025年第1期5-9,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金青年科学基金项目(编号:82004272);上海市松江区科学技术攻关项目(编号:22SJKJGG40);上海市松江区公共卫生协同能力提升项目(编号:SJGW6-24);上海市教育学会课题项目(编号:2QYB24108)。

摘　　要：目的探讨7,8-二羟基黄酮(7,8-DHF)对糖尿病大鼠视网膜的保护作用及其机制。方法取SPF级雄性SD大鼠18只,将大鼠随机分为正常组、模型组和实验组,每组6只。正常组采用普通饲料喂养,其余大鼠采用高脂乳剂连续灌胃2周建立糖尿病模型。实验组大鼠给予7,8-DHF(5 mg·kg^(-1))腹腔注射干预,正常组和模型组大鼠给予同等量生理盐水,各组大鼠每天干预1次,连续2周。观察各组大鼠造模前后体重与空腹血糖变化。2周后采用眼底照相和眼底荧光素血管造影(FFA)观察各组大鼠眼底视网膜变化。采用HE染色、CD31免疫荧光及TUNEL实验检测大鼠视网膜神经细胞变化及凋亡情况。结果干预2周后,与正常组相比,模型组和实验组大鼠体重均下降,空腹血糖均上升,差异均有统计学意义(均为P<0.05);与模型组相比,实验组大鼠体重上升,空腹血糖下降,差异均有统计学意义(P<0.05)。各组大鼠眼底照相和FFA检查均未发现糖尿病性视网膜病变眼底改变。HE染色结果显示,正常组和实验组大鼠视网膜结构完整,排列整齐,厚薄均匀,模型组大鼠视网膜层次尚清晰。与正常组及实验组相比,模型组大鼠视网膜内层厚度均变薄,差异均有统计学意义(均P<0.05)。CD31免疫荧光染色结果显示,各组大鼠CD31免疫荧光强度值大致相当,差异无统计学意义(均为P>0.05)。TUNEL实验结果显示,与正常组比较,模型组大鼠视网膜神经凋亡细胞数明显增加,差异有统计学意义(P<0.001);与模型组相比,实验组大鼠视网膜神经凋亡细胞数明显减少,差异有统计学意义(P<0.001)。结论糖尿病大鼠视网膜神经凋亡可能早于血管内皮细胞损伤。7,8-DHF可以改善糖尿病大鼠体重及降低血糖,保护DM大鼠视网膜神经细胞。Objective To explore the protective effect of 7,8-dihydroxyflavone(7,8-DHF)on the retina of diabetic rats and its mechanism.Methods A total of 18 SPF-grade male Sprague-Dawley(SD)rats were selected and randomly divided into three groups:the normal group,the model group,and the experimental group,with six rats in each group.Rats in the normal group were fed with a normal diet,while those in the remaining two groups were fed with a high-fat emulsion through oral gavage continuously for 2 weeks to establish a diabetes model.Rats in the experimental group were provided with 7,8-DHF(5 mg·kg^(-1))by continuous intraperitoneal injection,while those in the normal and model groups were provided with an equal volume of normal saline.The rats in all groups received intervention once a day for 2 weeks.The changes in the body mass and fasting blood glucose(FBG)were observed before and after modeling.Besides,the changes in the retina of rats in each group were observed by fundus fluorescence angiography(FFA)after 2 weeks.Moreover,the changes and apoptosis of retinal neuronal cells were detected by hematoxylin and eosin(HE)staining,CD31 immunofluorescence,and terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)assays.Results After 2 weeks of continuous intervention,compared with the normal group,the body mass of rats in the model and experimental groups decreased(both P<0.05),and the FBG increased significantly(both P<0.05);compared with the model group,the experimental group showed an increase in the body mass(P<0.05)and a decrease in the FBG(P<0.05).The fundus photography and FFA of rats in the three groups did not reveal any fundus features of diabetic retinopathy.The HE staining results showed that the retina of rats in the normal and experimental groups was structurally intact,with neatly arranged cells and uniform thickness;the retinal structure of rats in the model group remained clear.However,the thickness of the inner layers of the retina of rats in the model group was thinner compared with the normal a

关 键 词：7 8-二羟基黄酮 糖尿病大鼠 视网膜神经细胞 凋亡 

分 类 号：R774[医药卫生—眼科]