胞壁酰二肽对儿童急性白血病骨髓树突状细胞体外扩增的影响  

EFFECT OF MDP ON THE CULTURE AND PROLIFERATION OF DC DERIVED FROM BONE MARROW IN CHILDREN WITH AL IN VITRO

作　　者：孙绍霞[1] 刘春雷 王敏[1] 李晓玲 SUN Shao-xia;LIU Chun-lei;WANG Ming;LI Xiao-ling(Linyi City People's Hospital,Linyi,China 276000;Shandong Provincial Third Hospital,Linyi,China 250000)

机构地区：[1]临沂市人民医院,山东临沂276000 [2]山东省立第三医院,山东济南250000

出　　处：《山东医学高等专科学校学报》2024年第6期13-15,F0003,共4页Journal of Shandong Medical College

基　　金：山东省医药卫生科技发展计划项目(No.202206011117)。

摘　　要：目的探讨儿童AL的治疗方法。方法采用Ficoll-Hypaque法分离AL患儿MNC,将其分为4组。对照组:以含10%FCS的RPMI1640培养液培养;实验1组:单用MDP;实验2组:rhGM-CSF+rhIL-4+rhTNF-α;实验3组:rhGM-CSF+rhIL-4+rhTNF-α+MDP。观察每日细胞生长情况及细胞形态,培养8d后计数各组细胞数量并应用流式细胞仪检测各组细胞免疫表型。结果对照组培养3 d,细胞大量死亡,数量明显减少;而实验各组在培养第2-8天均出现细胞形态的变化;实验各组细胞数及HLA-DR、CD1a、CD83细胞比例均高于对照组,以实验3组最高(P<0.05)。结论MDP对AL患儿骨髓DC有扩增作用并促其成熟,与细胞因子联合作用更强。Objective To explore the treatment methods for children with AL.Methods The Ficoll Hypaque method was used to isolate MNCs from AL patients,who were divided into four groups.Control group:cultured in RPMI1640 medium containing 10%FCS;Experiment 1 group:using MDP alone;Experiment 2 group:rhGM-CSF+rhIL-4+rhTNF-α;Experiment 3 group:rhGM-CSF+rhIL-4+rhTNF-α+MDP.Observe daily cell growth and morphology,count the number of cells in each group after 8 days of culture,and use flow cytometry to detect the immune phenotype of each group of cells.Results After 3 days of cultivation in the control group,a large number of cells died and the quantity significantly decreased;And all experimental groups showed changes in cell morphology from day 2 to day 8 of cultivation;The number of cells and the proportion of HLA DR,CD1a,and CD83 cells in each experimental group were higher than those in the control group,with the highest in experimental group 3(P<0.05).Conclusion MDP has an amplifying effect on bone marrow DCs in children with AL and promotes their maturation,with a stronger synergistic effect with cytokines.

关 键 词：胞壁酰二肽 树突状细胞 急性白血病 骨髓 扩增 

分 类 号：R733.71[医药卫生—肿瘤]