大豆根瘤菌Q7-3菌株分离鉴定及特性分析  

Isolation,identification and characteristic analysis of soybean rhizobium Q7-3 strain

作　　者：朱加楠 毕宇 潘钰[1] 姜超 于冲[1] 王玉霞[1] 刘春燕 ZHU Jia′nan;BI Yu;PAN Yu;JIANG Chao;YU Chong;WANG Yuxia;LIU Chunyan(Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin 150010;Heilongjiang Academy of Sciences,Harbin 150001,China)

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010 [2]黑龙江省科学院,黑龙江哈尔滨150001

出　　处：《生物技术》2024年第6期722-727,共6页Biotechnology

基　　金：黑龙江省省属科研业务费项目(2023SSKY004-03)。

摘　　要：[目的]分离筛选黑龙江省齐农7号大豆株系根瘤菌,探究其基本特性及对齐农7号大豆生长结瘤的影响。[方法]组织分离法分离筛选根瘤菌,进行系统发育分析,明确其种属关系。回接盆栽试验确定根瘤菌对寄主结瘤能力的影响。液体培养测定根瘤菌耐盐碱能力,分析其对唯一碳氮源的利用能力。[结果]从齐农7号大豆中分离出1株根瘤菌Q7-3,经系统发育分析鉴定为快生型贾氏根瘤菌(Rhizobium giardinii)。将根瘤菌Q7-3回接到齐农7号大豆后,与对照相比,其叶绿素含量为29.37±0.53(SPAD值),提高了29.21%;氮素含量为(12.87±0.53)mg/g,提高了30.93%;根瘤数、根瘤鲜重分别为(17±5.72)个、(0.13±0.01)g。基本特性分析发现根瘤菌Q7-3不耐盐胁迫。当培养环境pH值≤10.31时,根瘤菌Q7-3在碱性环境中生长繁殖能力较强,pH值≥10.92时根瘤菌Q7-3几乎不生长。碳氮源利用测定发现,根瘤菌Q7-3能以蔗糖作为唯一碳源。以硫酸铵、胰蛋白胨和尿素为唯一氮源时,根瘤菌Q7-3几乎不生长或繁殖能力较弱。[结论]根瘤菌Q7-3为快生型贾氏根瘤菌且可促进齐农7号大豆结瘤,有耐碱特性,能以蔗糖为唯一碳源生长繁殖。[Objective]To isolate,screen and identify rhizobium in Qinong 7 soybean of Heilongjiang Province,and study the effects of rhizobium on the growth and nodulation of Qinong 7 soybean.[Method]Rhizobium was isolated and screened by tissue isolation,and the phylogenetic analysis of rhizobium was carried out to clarify its species relationship.The effect of rhizobium on host nodulation ability was determined by tieback pot experiment.The salt-alkali tolerance of rhizobium was measured by liq-uid culture,and its utilization of the only carbon and nitrogen source was analyzed.[Result]A strain of rhizobium Q7-3 was isolated from Qinong 7 soybean and identified as Rhizobium giardinii by phylogenetic analysis.Compared with the control,the chlorophyll content of rhizobium Q7-3 was 29.37±0.53(SPAD value),which was significantly increased by 29.21%.Nitro-gen content was(12.87±0.53)mg/g,significantly increased by 30.93%;The number and fresh weight of root nodules were(17±5.72)and(0.13±0.01)g,respectively.The basic characteristics of rhizobium Q7-3 were analyzed and it was found that was not tolerant to salt stress.However,when the pH value in the culture environment was lower than 10.31,the growth and reproduction ability of rhizobium Q7-3 in the alkaline environment was still strong,but when the pH value was adjusted to 10.92,rhizobium Q7-3 hardly grew.Through the determination of carbon and nitrogen source utilization of rhizobium Q7-3,it was found that sucrose could be used as the only carbon source to provide reproductive nutrition for rhizobium Q7-3.When ammonium sulfate,tryptone and urea were the only nitrogen sources,rhizobium Q7-3 hardly grew or had weak reproductive ca-pacity.[Conclusion]Rhizobium Q7-3 is a fast growing rhizobium,which can promote nodulation of Qinong 7 soybean,and it has alkali resistance characteristics,and can effectively use sucrose as the only carbon source for growth and reproduction.

关 键 词：大豆 根瘤菌 共生 结瘤 耐盐性 耐碱性 碳源 氮源 

分 类 号：Q784[生物学—分子生物学]