miR-217调控NRP1对胃癌细胞奥沙利铂耐药能力的影响  

Effect of miR-217 on oxaliplatin resistance of gastric cancer cells by regulating NRP1

作　　者：周颖[1] 许琦华 廖冰灵[1] 常玲 张旗林[2] ZHOU Ying;XU Qihua;LIAO Bingling;CHANG Ling;ZHANG Qilin(Department of Gastroenterology,The Seventh People′s Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200136;Department of General Surgery,The Seventh People′s Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200136,China)

机构地区：[1]上海中医药大学附属第七人民医院消化科,上海200136 [2]上海中医药大学附属第七人民医院普外科,上海200136

出　　处：《生物技术》2024年第6期728-734,共7页Biotechnology

基　　金：浦东新区科技发展基金事业单位民生科研专项(PKJ2022-Y11)。

摘　　要：[目的]探索miR-217与NRP1在奥沙利铂耐药性胃癌细胞中的作用机制。[方法]将MGC803/L-OHP细胞分为4组:miR NC组、miR-217 mimic组、si NC组与si NRP1组。通过实时荧光定量PCR实验和蛋白免疫印迹实验检测耐药和非耐药细胞中miR-217和NRP1的表达水平;通过MTT实验分析MGC803/L-OHP细胞的增殖能力;通过Transwell实验分析MGC803/L-OHP细胞的转移活性;通过流式细胞术分析MGC803/L-OHP细胞的凋亡率;通过荧光素酶报告基因实验分析MGC803/L-OHP细胞中miR-217与NRP1的靶向关系。[结果]上调miR-217或抑制NRP1能够抑制非耐药MGC803细胞生长。与MGC803细胞比较,MGC803/L-OHP细胞中的miR-217表达水平降低(0.79±0.08 vs 0.21±0.02;P<0.05),NRP1表达水平增加(0.41±0.09 vs 0.78±0.12;P<0.05)。与miR NC组比较,miR-217 mimic组的MGC803/L-OHP细胞的增殖活性降低、侵袭能力降低、凋亡率增加(2.37±0.23 vs 25.38±5.12;P<0.05)。与si NC组比较,si NRP1组的MGC803/L-OHP细胞的增殖活性降低、侵袭能力降低、凋亡率增加(4.67±0.83 vs 26.17±7.19;P<0.05)。荧光素酶活性测定显示,和miR NC组比较,在NRP1-WT组中转染miR-217 mimic后荧光素酶活性显著降低(0.87±0.03 vs 0.26±0.02;P<0.05)。[结论]在奥沙利铂耐药性胃癌细胞中,miR-217低表达而NRP1高表达,并且miR-217能够靶向抑制NRP1的表达。通过上调miR-217或抑制NRP1的表达能够减弱胃癌细胞对奥沙利铂的耐药性。[Objective]To explore the mechanism of miR-217 and NRP1 in oxaliplatin-resistant gastric cancer cells.[Method]MGC803/L-OHP cells were divided into four groups:miR NC group,miR-217 mimic group,si NC group and si NRP1 group.The expression levels of miR-217 and NRP1 in drug-resistant and non-drug-resistant cells were detected by real-time fluorescent quantitative PCR and Western Blot.The proliferation ability of MGC803/L-OHP cells was analyzed by MTT assay.Transwell assay was used to analyze the metastatic activity of MGC803/L-OHP cells.The apoptosis rate of MGC803/L-OHP cells was analyzed by flow cytometry.Luciferase reporter assay was used to analyze the targeting relationship between miR-217 and NRP1 in MGC803/L-OHP cells.[Result]Up-regulation of miR-217 or inhibition of NRP1 could inhibit the growth of non-drug resistant MGC803 cells.Compared with MGC803 cells,the expression level of miR-217 in MGC803/L-OHP cells was decreased(0.79±0.08 vs 0.21±0.02;P<0.05)and increased NRP1 expression(0.41±0.09 vs 0.78±0.12;P<0.05).Compared with the miR NC group,the proliferation activity and invasion ability of MGC803/L-OHP cells in the miR-217 mimic group were decreased,and the apoptosis rate was increased(2.37±0.23 vs 25.38±5.12;P<0.05).Compared with the si NC group,the proliferation activity and invasion ability of MGC803/L-OHP cells in the si NRP1 group were decreased,and the apoptosis rate was increased(4.67±0.83 vs 26.17±7.19;P<0.05).Luciferase activity assay showed that compared with miR NC group,the luciferase activity was significantly decreased in NRP1-WT group after transfection with miR-217 mimic(0.87±0.03 vs 0.26±0.02;P<0.05).[Conclusion]miR-217 is down-regulated and NRP1 is up-regulated in oxaliplatin-resistant gastric cancer cells,and miR-217 can target and inhibit the expression of NRP1.The oxaliplatin resistance of gastric cancer cells can be inhibited by up-regulation of miR-217 or inhibition of NRP1 expression.

关 键 词：miR-217 NRP1 奥沙利铂 胃癌 耐药 侵袭 MGC803 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]