miR-612调控FOXP1影响白血病HL-60细胞的转移活性  

miR-612 regulates the metastatic activity of leukemia HL-60 cells by regulating FOXP1

作　　者：胡灵丹 杜立坤 刘影 杜志强[2] 崔菁 HU Lingdan;DU Likun;LIU Ying;DU Zhiqiang;CUI Jing(Department of Pathology,The Second People′s Hospital of Hengshui,Hengshui 053000;Department of Hematology,The Second People′s Hospital of Hengshui,Hengshui 053000;Molecular Laboratory,The Second People′s Hospital of Hengshui,Hengshui 053000,China)

机构地区：[1]衡水市第二人民医院病理科,河北衡水053000 [2]衡水市第二人民医院血液科,河北衡水053000 [3]衡水市第二人民医院分子实验室,河北衡水053000

出　　处：《生物技术》2024年第6期742-748,共7页Biotechnology

基　　金：衡水市科技计划项目(2022014048Z)。

摘　　要：[目的]探究miR-612与FOXP1对急性髓系白血病(AML)细胞活性的影响。[方法]通过免疫组化实验分析正常组和AML组的骨髓样本中的FOXP1表达水平。将人急性髓系白血病细胞HL-60细胞分为4组:miR NC组、miR-612 mimic组、si NC组和si FOXP1组。通过MTT实验检测细胞生长能力,实时荧光定量PCR实验检测miR-612和FOXP1表达水平,Transwell实验分析细胞迁移能力,蛋白免疫印迹实验分析FOXP1表达水平,流式细胞术分析细胞凋亡率。[结果]与正常组骨髓样本相比,AML组骨髓样本miR-612表达水平降低(P<0.05),而FOXP1的表达水平增加(P<0.05)。与miR NC组比较,miR-612 mimic组HL-60细胞的增殖能力和转移能力显著降低,凋亡率增加(P<0.05)。与si NC组比较,si FOXP1组HL-60细胞的增殖能力和转移能力显著降低,凋亡率增加(P<0.05)。荧光素酶报告基因结果显示,miR-612能够靶向调控FOXP1表达(P<0.05)。[结论]上调miR-612表达能够抑制HL-60细胞的增殖能力和转移能力,并能促进HL-60细胞凋亡。此外,miR-612对HL-60细胞的这一作用与靶向抑制FOXP1表达有关。[Objective]To investigate the effect of miR-612 and FOXP1 on the activity of acute myeloid leukemia cells.[Method]The expression levels of FOXP1 in normal and AML bone marrow samples were analyzed by immunohistochemistry.Human acute myeloid leukemia HL-60 cells were divided into four groups:miR NC group,miR-612 mimic group,si NC group and si FOXP1 group.Cell growth was measured by MTT assay.The expression levels of miR-612 and FOXP1 were de-tected by real-time fluorescence quantitative PCR.Cell migration ability was analyzed by Transwell assay.The expression level of FOXP1 was analyzed by Western Blot.The apoptosis rate was analyzed by flow cytometry.[Result]Compared with normal bone marrow samples,the expression level of miR-612 in bone marrow samples of AML patients decreased(P<0.05),while the expression level of FOXP1 increased(P<0.05).Compared with the miR NC group,the proliferation and metastasis ability of HL-60 cells in the miR-612 mimic group were significantly decreased,and the apoptosis rate was increased(P<0.05).Compared with the si NC group,the proliferation and metastasis ability of HL-60 cells in the si FOXP1 group were significant-ly decreased,and the apoptosis rate was increased(P<0.05).Luciferase reporter gene results showed that miR-612 could target and regulate the expression of FOXP1(P<0.05).[Conclusion]Up-regulation of miR-612 expression can inhibit the proliferation and metastasis of HL-60 cells,and promote the apoptosis of HL-60 cells.In addition,the effect of miR-612 on HL-60 cells was related to the inhibition of FOXP1 expression.

关 键 词：miR-612 急性髓系白血病 FOXP1 转移 侵袭 HL-60 凋亡 增殖 

分 类 号：R733.7[医药卫生—肿瘤]