MUC16硫酸化O型连接糖促进食管鳞癌细胞迁移  

Promotion of migration of esophageal squamous carcinoma cells by sulfated O-linked glycans of MUC16

作　　者：周珍[1] 魏娉[1] 姜琳娜[1] 何鹏 尹峰[1] 安欣[1] ZHOU Zhen;WEI Ping;JIANG Linna;HE Peng;YIN Feng;AN Xin(Department of Pathology,Handan First Hospital,Handan 056002,China)

机构地区：[1]邯郸市第一医院病理科,河北邯郸056002

出　　处：《生物技术》2024年第6期761-766,共6页Biotechnology

基　　金：河北省卫生健康委员会项目(20231903)。

摘　　要：[目的]探讨MUC16蛋白的硫酸化O型连接糖对食管鳞癌细胞的迁移作用。[方法]TCGA数据库分析MUC16在食管癌组织中的表达水平。过表达或敲低MUC16后,划痕实验检测食管鳞癌细胞KYSE-30的迁移水平。过表达或敲低CHST4后,Lectin blot和Alcian blue染色检测食管鳞癌细胞中MUC16的O型连接糖硫酸化水平以及食管鳞癌细胞的迁移水平,LC-ESI-MS检测MUC16的O型连接糖类型。[结果]相比于食管正常组织,食管癌组织中MUC16和CHST4的表达水平较高(0.78±0.13 vs 0.09±0.02;0.43±0.08 vs 0.07±0.02,P<0.05)。过表达MUC16后,食管鳞癌细胞的迁移水平显著上升;敲低MUC16后,食管鳞癌细胞的迁移水平显著下降。敲低CHST4后,MUC16 O型连接糖的硫酸化水平显著下降,食管鳞癌细胞的迁移水平显著下降;过表达CHST4后,MUC16 O型连接糖的硫酸化水平显著上升,食管鳞癌细胞的迁移水平显著上升。食管鳞癌细胞在m/z 813和1121处发现了含有GlcNAc-6-O-硫酸的低聚糖,而敲低CHST4后硫酸化的低聚糖水平降低。[结论]CHST4介导的MUC16蛋白O型连接糖硫酸化对食管鳞癌细胞的迁移具有促进作用(1.00±0.05 vs 1.83±0.12)。[Objective]To investigate the migratory effect of sulfated O-linked glycans of MUC16 protein on esophageal squa-mous carcinoma cells.[Method]TCGA database was analyzed for the expression level of MUC16 in esophageal cancer tissues.After overexpression or knockdown of MUC16,the migration level of KYSE-30 in esophageal squamous carcinoma cells was detected by scratch assay.After overexpression or knockdown of CHST4,Lectin blot and Alcian blue staining were performed to detect the sulfation level of O-linked glycans of MUC16 protein in esophageal squamous carcinoma cells and the migration lev-el of esophageal squamous carcinoma cells,and LC-ESI-MS was performed to detect the O-linked glycan type of MUC16 protein.[Result]Compared with normal esophageal tissues,the expression level of MUC16 and CHST4 in esophageal cancer tissues was higher(0.78±0.13 vs 0.09±0.02,P<0.05)and the expression level of CHST4 was higher(0.43±0.08 vs 0.07±0.02,P<0.05).After overexpression of MUC16,the migration level of esophageal squamous cell cells increased significant-ly.After MUC16 was knocked down,the migration level of esophageal squamous cell cells decreased significantly.After CHST4 was knocked down,the sulfation level of MUC16 O-type linked sugar was significantly decreased,and the migration level of e-sophageal squamous cell carcinoma was significantly decreased.After overexpression of CHST4,the sulfation level of MUC16 O-type link-sugar was significantly increased,and the migration level of esophageal squamous cell carcinoma was significantly increased.Glcacc-6-O-sulfuric acid oligosaccharides were found in esophageal squamous cell cells at m/z 813 and 1121,while sulfated oligosaccharides were reduced after CHST4 knockdown.[Conclusion]Chst4-mediated MUC16 protein O-type link-sugar sulfation promoted the migration of esophageal squamous cell cells(1.00±0.05 vs 1.83±0.12).

关 键 词：MUC16 硫酸化 O型连接糖 食管鳞癌细胞 迁移 CHST4 GlcNAc-6-O-硫酸 KYSE-30 

分 类 号：R735.1[医药卫生—肿瘤]