胶体金免疫层析法快速检测动物源食品中的诺氟沙星  

Rapid Detection of Norfloxacin in Animal-Derived Foods by Colloidal Gold Immunochromatography

作　　者：陈文硕 温宇浩 娄婷婷 潘红蕊 李金花[2] CHEN Wen-shuo;WEN Yu-hao;LOU Ting-ting;PAN Hong-rui;LI Jin-hua(Industrial Product Safety Technology Center,Tianjin Customs,Tianjin 300457,China;Shandong Key Laboratory of Coastal Environmental Processes,Coastal Zone Ecological Environment Monitoring Technology and Equipment Shandong Engineering Research Center,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;School of Bioengineering,Tianjin University of Science and Technology,Tianjin 300222,China)

机构地区：[1]天津海关工业产品安全技术中心,天津300457 [2]中国科学院烟台海岸带研究所海岸带生态环境监测技术与装备山东省工程研究中心山东省海岸带环境过程重点实验室,山东烟台264003 [3]天津科技大学生物工程学院,天津300222

出　　处：《化学试剂》2025年第1期92-96,共5页Chemical Reagents

基　　金：海关总署课题项目(2023HK084);天津市科技支撑重点项目(20YFZCSN00630);国家自然科学基金项目(22176210);山东省自然科学基金项目(ZR2020KC032)。

摘　　要：胶体金免疫层析法具有高特异性、高灵敏度、高重复性等特点,能够准确地检测出样品中的目标物,在快速检测领域具有广泛应用。本研究将胶体金免疫层析法用于快速检测动物源食品(猪肉、鸡肉、鱼肉、虾肉、鸡蛋)中的诺氟沙星(Norfloxacin,NOR)残留,对方法进行验证,相对准确度为97.0%。方法的检出限为2μg/kg,检测性能强。在上述5种不同基质样品中,NOR胶体金快速检测方法的灵敏度为99%、特异度为98%、假阳性率为2%、假阴性率为1%。该方法简单快速、灵敏准确,适于复杂基质中NOR的快速精准检测。Colloidal gold immunochromatography has the characteristics of high specificity,high sensitivity,and high repeatability,which can accurately detect the targeted analytes in the samples,and has a wide range of applications in the field of rapid detection.This study used colloidal gold immunochromatography to rapidly detect norfloxacin(NOR)residues in animal-derived foods(pork,chicken,fish,shrimp,and eggs)and validated the method with a relative accuracy of 97.0%.The limit of detection was down to 2μg/kg,showing high detectability.By testing the above-mentioned five different matrix samples,the NOR colloidal gold rapid detection method attained a sensitivity of 99%,specificity of 98%,false positive rate of 2%,and false negative rate of 1%.This method proved simple,rapid,sensitive,and accurate,and thereby was practically applicable for the rapid,precise detection of NOR in complicated matrices.

关 键 词：胶体金免疫层析法 诺氟沙星 方法验证 动物源食品 快速检测 

分 类 号：O657.31[理学—分析化学]