5株牛源奇异变形杆菌致病性及药物敏感性分析  

Pathogenicity and Drug Sensitivity Analysis of Proteus Mirabilis from Five Bovine Proteus mirabilis Strains

作　　者：但蕊桐 谢沐含 邱羊羊 陈建宇 李能章[1] 彭远义[1] DAN Ruitong;XIE Muhan;QIU Yangyang;CHEN Jianyu;LI Nengzhang;PENG Yuanyi(College of Veterinary Medicine,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学动物医学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2025年第2期26-40,共15页Journal of Southwest University(Natural Science Edition)

基　　金：国家现代农业(肉牛牦牛)产业技术体系建设专项(CARS-37)。

摘　　要：为探究牛源奇异变形杆菌的致病性及药物敏感性差异,对前期分离到的5株试验菌株(SPL569、SPL1502、SPL1504、SPL1511、SPL1512)进行了进一步的常规细菌学鉴定及群集运动分析、16S rDNA序列比对、动物致病性试验和毒力基因检测,通过药物敏感性试验和耐药基因检测分析其耐药性。结果显示:5株试验菌株均为奇异变形杆菌,均具有在1.5%琼脂平板上发生同心圆迁移的群集运动现象,其中SPL1511和SPL1512迁移速度快于其他菌株,群集运动能力更强。致病性试验表明:只有SPL1511和SPL1512两株试验菌对小鼠致死。毒力基因检测发现:SPL1511和SPL1512具有特殊的“雾蔓”迁徙能力调节因子(rsbA),验证了该基因是拥有致病性的关键,同时也验证了群集运动能力越强、其侵袭力也越强。药物敏感性试验结果表明:5株试验菌对临床常见的大部分抗生素基本耐药,其中SPL569、SPL1502、SPL1504菌株只对头孢曲松敏感,SPL1511只对头孢他定敏感,SPL1512只对头孢哌酮敏感。耐药基因检测结果也与药物敏感性试验结果相符合。In order to explore the differences in pathogenicity and drug resistance among five bovine Proteus mirabilis strains,In this study,the five strains(SPL569,SPL1502,SPL1504,SPL1511,SPL1512)isolated previously were further identified by routine bacteriological analysis,swarming motility analysis,16S rDNA sequence alignment,animal pathogenicity test and virulence factor detection.Drug resistance was analyzed by drug sensitivity test and drug resistance gene detection.The results showed that the 5 strains were all Proteus mirabilis,and all had concentric circle migration on a 1.5%agar plate.SPL1511 and 1512 migrated faster than other strains and had stronger swarming motility.The results of the pathogenicity test showed that only SPL1511 and SPL1512 were lethal to mice.Virulence gene detection showed that only SPL1511 and SPL1512 had unique rsbA gene.It not only confirmed that the migration ability regulatory factor(rsbA)gene is the key to the pathogenicity,but also verified that the stronger the swarming motility ability,the stronger the invasiveness.The results of the drug sensitivity test showed that the 5 strains were basically resistant to most common antibiotics in clinical practice,among which SPL569,SPL1502,and SPL1504 strains were only sensitive to ceftriaxone,SPL1511 was only sensitive to ceftazidim,and SPL1512 was only sensitive to cefoperazone.The results of drug resistance gene detection were also consistent with the results of drug sensitivity test in this study.

关 键 词：奇异变形杆菌 群集运动 致病性 毒力基因 耐药性 耐药基因 

分 类 号：S855[农业科学—临床兽医学]