基于HS-SPME-GC-MS解析核桃雄花挥发性成分  

Analysis the Volatile Components of Walnut Male Flower(Juglans sigillata)Based on HS-SPME-GC-MS

作　　者：王纪辉 胡伯凯 周雯 WANG Jihui;HU Bokai;ZHOU Wen(Guizhou Institute of Walnut/Guizhou Province Walnut Engineering and Technology Research Center,Guiyang 550005,China;Guizhou Public Resources Trading Center,Guiyang 550002,China Abstract:)

机构地区：[1]贵州省核桃研究所/贵州省核桃工程技术研究中心,贵阳550005 [2]贵州省公共资源交易中心,贵阳550002

出　　处：《西南大学学报（自然科学版）》2025年第2期51-60,共10页Journal of Southwest University(Natural Science Edition)

基　　金：贵州省科技计划项目(黔科合支撑〔2022〕重点014号,黔科合平台人才〔2019〕5202号);贵州省林业科研项目(黔林科合J字〔2024〕11号);贵州省木本粮油加工科技创新人才团队项目(黔科合平台人才〔2020〕5003)。

摘　　要：检测分析核桃雄花的香气组分种类、相对体积分数,探明核桃雄花的呈香成分及香气特征。以鲜核桃雄花为材料,采用顶空固相微萃取串联气质技术(HS-SPME-GC-MS)对核桃雄花中挥发性成分进行鉴定和定量分析。结果从核桃雄花中共鉴定出22种挥发性成分,其中胡桃醌、二甲硫醚2种化合物占总体积分数的62.18%±2.93%、醇类占19.03%±1.91%、烯烃类占10.32%±1.84%、醛类、酮类及酯类化合物占比较低;核桃雄花香气主要由芳樟醇、反松香芹醇、2-甲基丙醛、2-甲基丁醛、3-甲基丁醛、壬醛、十六烷酸乙酯及烯烃类所贡献;主成分分析将22种挥发性化合物归为2类。综合显示:贵州产区核桃雄花香气主要由醇类、醛类和烯烃类成分所构成,种类最为丰富的烯烃类化合物贡献了突出的水果类气味。The types and relative content of aroma components of the walnut(Juglans sigillata)male flowers were detected and analyzed to explore the fragrant composition and aroma characteristics of walnut male flowers.Walnut male flower of Guizhou region was used as the research object.We applied head space solid phase microextraction coupled with gas chromatography-mass spectrometry(HS-SPME-GC-MS)to analyze and identify volatile substances.22 volatile components were identified from walnut male flowers,among which two compounds of juglone and dimethyl sulfide accounted for 62.18%±2.93%of the total content,and alcohols and olefin accounted for 19.03%±1.91%and 10.32%±1.84%of total content respectively,while the contents of aldehydes,ketones and esters were low.The aroma of walnut male flowers is mainly contributed by linalool,trans-pinocarveol,2-methyl-propanal,2-methyl butanal,3-methyl butanal,nonanal,palmitic acid ethyl ester and olefin compounds.Principal component analysis classified 22 volatile compounds into the 2 categories.The aroma volatiles of walnut male flowers in Guizhou region was mainly composed of alcohols,aldehydes and alkenes.The most abundant olefin compounds species contributed to the prominent fruit odor.

关 键 词：核桃雄花 挥发性成分 顶空固相微萃取串联气质技术 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]