不同提取方法所得桢楠木提取物化学成分分析  

Chemical Composition Analysis of Extracts From Phoebe zhennan Wood Obtained by Different Extraction Methods

作　　者：孙龙菲 宁莉萍[1,2] 黄建 余慧茹 蒋容 徐艺萌 SUN Longfei;NING Liping;HUANG Jian;YU Huiru;JIANG Rong;XU Yimeng(College of Forestry,Sichuan Agricultural University,Chengdu 610000,China;Wood Industry and Furniture Engineering of Sichuan University Key Laboratory,Sichuan Agricultural University,Chengdu 611130,China;Sichuan Bluetown Testing Technology Co.,Ltd.,Chengdu 610200,China)

机构地区：[1]四川农业大学林学院,四川成都610000 [2]四川农业大学木材工业与家具工程四川省高校重点实验室,四川成都611130 [3]四川蓝城检测技术有限公司,四川成都610200

出　　处：《林产化学与工业》2024年第6期103-110,共8页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(32201281)。

摘　　要：以桢楠木为研究对象,分别采用水蒸气蒸馏法(SD)、超临界CO_(2)萃取法(SFE)、乙醇提取法(EE)、乙酸乙酯提取法(EAE)和正己烷提取法(HE)这5种方法得到桢楠木提取物,通过气相色谱-质谱联用(GC-MS)技术对比其化学成分的差异,并用高效液相色谱(HPLC)法定量检测其主要化学成分。研究结果表明:5种方法得到的提取物中共鉴定出132种化合物,相对峰面积之和介于88.49%~97.16%之间,化学成分主要包括酯类、醇类、烃类、烯类、酸类、醛类、酮类、酚类这8大类;不同提取方法所得的桢楠木提取物中,共有化合物是醇类、烯类、酮类3大类,其中醇类化合物是所有提取物中的重要组成部分。在5种方法得到的桢楠木提取物中,沉香螺醇和缬草醇的相对峰面积之和为13.73%~29.43%,两者的含量之间存在极显著的正相关关系,且由于两者化学结构相似,所含极性基团相同,在色谱上的保留行为基本一致,导致分离难度较大。采用超临界CO_(2)萃取法提取时,沉香螺醇和缬草醇的得率最高,分别为0.305%和0.157%;水蒸气蒸馏法次之,溶剂提取法最低。Taking Phoebe zhennan S.K.Lee&F.N.Wei as the research object,the P.zhennan extracts were obtained by the five methods of steam distillation(SD)method,supercritical CO_(2)extraction(SFE)method,ethanol extraction(EE)method,ethyl acetate extraction(EAE)method and n-hexane extraction(HE)method.The differences of chemical composition of P.zhennan were compared by gas chromatography and mass spectrometry(GC-MS),and the main chemical components were quantitatively determined by high performance liquid chromatography(HPLC).The results showed that a total of 132 compounds were identified in the extracts obtained by the five methods,and the sum of their relative peak areas ranged from 88.49%to 97.16%.The chemical components mainly included esters,alcohols,hydrocarbons,alkanes,acids,aldehydes,ketones and phenols.Among the P.zhennan extracts obtained by different extraction methods,there were three kinds of common compounds:alcohols,alkenes and ketones,where alcohols were the important part of all extracts.Agarospirol and valerianol were decisive compounds in all P.zhennan extracts.The sum of the relative peak areas of P.zhennan extracts by the five methods ranged from 13.73%to 29.43%,and there was a very significant positive correlation between them.Due to their similar chemical structures and the same polar groups,the retention behavior of the two compounds in the chromatography was basically the same,and the separation was difficult.By supercritical CO_(2)extraction method,the yields of agarospirol and valerianol were the highest,0.305%and 0.157%,respectively,followed by steam distillation method,and solvent extraction method was the lowest.

关 键 词：提取方法 化学成分 得率 

分 类 号：TQ35[化学工程] Q946.85[生物学—植物学]