结直肠腺癌组织PRRX1蛋白表达及其与微卫星不稳定性的关系分析  

Expression of PRRX1 Protein in Colorectal Adenocarcinoma Tissue and Its Relationship with Microsatellite Instability

作　　者：谷心语 GU Xin-yu(Department of Pathology,The First Affiliated Hospital of Nanyang Medical College,Nanyang 473007,Henan Province,China)

机构地区：[1]南阳医学高等专科学校第一附属医院病理科,河南南阳473007

出　　处：《罕少疾病杂志》2025年第1期130-132,共3页Journal of Rare and Uncommon Diseases

摘　　要：目的探讨结直肠腺癌组织配对相关同源框1(PRRX1)蛋白表达及其与微卫星不稳定性(MSI)的关系。方法回顾性分析本院2020年10月至2022年12月收治的168例结直肠腺癌患者的临床资料,均于术中留取结直肠腺癌组织与癌旁正常组织(距癌≥5cm)。采用免疫组织化学法检测结直肠腺癌组织和相应癌旁正常组织中PRRX1蛋白表达,并分析PRRX1蛋白表达与临床病理特征的关系。采用PCR法检测结直肠腺癌组织MSI情况,并将其分为微卫星高度不稳定(MSI-H)、微卫星低度不稳定(MSI-L)、微卫星稳定(MSS)3组,比较PRRX1蛋白在3组中.的表达情况。采用Spearman分析结直肠腺癌患者PRRX1蛋白表达与MSI的关系。结果结直肠腺癌组织中PRRX1蛋白阳性表达率高于癌旁正.常组织(P<005)。低分化、临床分期为Ⅲ+Ⅳ期、淋巴结转移患.者的PRRX1蛋白阳性表.达率高于中高分化、临.床分期为Ⅰ+Ⅱ期、无淋巴结转移患者(P<005)。16.8例结直肠腺癌患者中,经检测判定为MSS120例(7143%),MSI-H28例.(1667%).,MSI-L20例(1190%);MSI-H组PRRX1阳性表达率高于MSS组(P<005);经Spearman分析结果显示,PRRX1蛋白表达与MSI呈正相关(r=0568,P=0023)。结论PRRX1蛋白在结直肠腺癌组织中高表达,与患者肿瘤分化程度、临床分期、是否存在淋巴结转移有关,且与患者MSI的发生密切相关。Objective To investigate the expression of pairing-related homeobox 1(PRRX1)protein in colorectal adenocarcinoma tissue and its relationship with microsatellite instability(MSI).Methods The clinical data of 168 patients with colorectal adenocarcinoma admitted to our hospital from October 2020 to December 2022 were retrospectively analyzed.Colorectal adenocarcinoma tissues and normal tissue adjacent to the cancer(≥5cm away from cancer)were collected during operation.The expression of PRRX1 protein in colorectal adenocarcinoma tissues and corresponding normal tissue adjacent to the cancer was detected by immunohistochemistry,and the relationship between PRRX1 protein expression and clinicopathological features was analyzed.The MSI of colorectal adenocarcinoma tissues was detected by PCR and divided into three groups of microsatellite high instability(MSI-H),microsatellite low instability(MSI-L)and microsatellite stability(MSS).The expression of PRRX1 protein among the three groups was compared.Spearman was used to analyze the relationship between PRRX1 protein expression and MSI in patients with colorectal adenocarcinoma.Results The positive expression rate of PRRX1 protein in colorectal adenocarcinoma tissues was higher than that in normal tissue adjacent to the cancer(P<0.05).The positive expression rates of PRRX1 protein in patients with low differentiation,clinical stage III+IV and lymph node metastasis were higher than those in patients with medium and high differentiation,clinical stage I+II and no lymph node metastasis(P<0.05).Among 168 patients with colorectal adenocarcinoma,120 cases(71.43%)were diagnosed as MSS,and 28 cases(16.67%)as MSI-H and 20 cases(11.90%)as MSI-L.The positive expression rate of PRRX1 in MSI-H group was higher than that in MSS group(P<0.05).Spearman analysis showed that PRRX1 protein expression was positively correlated with MSI(r=0.568,P=0.023).Conclusion PRRX1 protein is highly expressed in colorectal adenocarcinoma tissues,which is related to the degree of tumor differentiation,c

关 键 词：结直肠腺.癌 配对相关同源框1 微卫星不稳定性 相关性 

分 类 号：R735.37[医药卫生—肿瘤]