柽柳根际土壤链霉菌Streptomyces sp.TRM76323基因簇分析及代谢产物分离  

Gene cluster analysis and metabolic product isolation of Streptomyces sp.TRM76323 in the rhizosphere soil of Tamarix

作　　者：陈乙煌 任佳 杨浩杰 李缘卓 王建明[1,2] 罗晓霞 Chen Yihuang;Ren jia;Yang Haojie;Li Yuanzhuo;Wang Jianming;Luo Xiaoxia(College of Life Science and Technology,Tarim Basin University,Alar 843300;State Key Laboratory for Biological Resources Protection and Utilization in the Tarim Basin,Alar 843300)

机构地区：[1]塔里木大学生命科学与技术学院,阿拉尔843300 [2]塔里木盆地生物资源保护利用省部共建国家重点实验室,阿拉尔843300

出　　处：《中国抗生素杂志》2024年第11期1281-1288,共8页Chinese Journal of Antibiotics

基　　金：国家自然科学基金项目(No.32360009);“西部之光”青年学者项目(No.2022-XBQNXZ-019);产膦酸类组分生防菌剂的制备与应用(No.TDZKZD202202);塔里木大学博创项目(No.TDBSCX202302);大学生创新创业项目(No.202210757017)。

摘　　要：目的利用链霉菌Streptomyces sp.TRM76323的基因组序列信息,分析其次级代谢产物生物合成基因簇并预测其代谢产物,为发现潜在新抗生素奠定基础。方法采用Illumina Novaseq对TRM76323菌株进行全基因组测序,结合antiSMASH、CARD、OrthoFinder、Peppan软件进行基因组分析,进行代谢潜力、抗性基因和基因组差异预测,为指导新的微生物来源的天然产物的发现提供重要的理论指导,并利用现代分离技术获得代谢产物。结果TRM76323菌株的基因组序列由7,338,911 bp组成,G+C含量为72.1%,共31个生物合成基因簇,有糖肽类耐药等抗性基因、分离获得化合物β-羰基-1H-吲哚-3-丙醛、环二肽和邻苯二甲酸二丁酯。结论柽柳根际土壤链霉菌Streptomyces sp.TRM76323有丰富的次级代谢产物生物合成基因簇,能产生多种次级代谢产物,具有进一步发掘新抗生素的价值。Objective This study analyzed the secondary metabolite biosynthesis gene clusters of Streptomyces sp.TRM76323 on the basis of its genomic sequence information,laying the foundation for the discovery of potential new antibiotics.Method The whole genome sequencing of the strain TRM76323.was performed by Illumina Novaseq.Additionally,antiSMASH,CARD,OrthoFinder and Pepperan softwares were used for genome analysis to predict metabolic potential,resistance genes,and genomic differences.These findings provided important theoretical guidance for the discovery of new natural products from microbial sources.Furthermore,modern isolation techniques were utilized to obtain metabolites.Results The genome sequence of the TRM76323 strain consisted of 7,338,911-base pairs,with a G+C content of 72.1%.A total of 31 biosynthetic gene clusters were identified,including resistance genes such as glycopeptide resistance.β-Carbonyl-1H-indole-3-propanal,cyclic dipeptide,and dibutyl phthalate were isolated from this strain.Conclusion Streptomyces sp.TRM76323,found in the rhizosphere soil of Tamarix,possessed a diverse clusters of secondary metabolite biosynthesis genes.This strain had the potential to produce various secondary metabolites and was worth further exploration for the discovery of new antibiotics.

关 键 词：柽柳根际 链霉菌 全基因组测序 次级代谢产物 

分 类 号：R978.1[医药卫生—药品] Q939.1[医药卫生—药学]