脑缺血-再灌注后海马迟发性神经元死亡的实验研究  

Experimental studies of delayed neuronal death following cerebral ischemia-reperfusion in mice

作　　者：蔡竖平[1] 桂秋萍[2] 韩志涛[1] 罗平[1] 王鲁宁[1] 

机构地区：[1]解放军总医院老年医学研究所,北京100853 [2]解放军总医院病理科,北京100853

出　　处：《军医进修学院学报》2002年第4期268-270,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的 :用重复脑缺血 再灌注小鼠模型的海马切片观察迟发性神经元死亡的形态学特点。方法 :用重复双侧颈总动脉夹闭方法制作不完全脑缺血 再灌注动物模型。于造模后 2 4h、72h、7d取材 ,HE染色及原位DNA末端标记法观察海马切片的病理改变。结果 :造模后 7d ,海马锥体细胞出现广泛的细胞坏死 ,以CA1区最明显。使用原位DNA末端标记法 ,在坏死神经元的邻近部位以及CA2 ,CA3区 ,齿状回可见部分膜结构完整的神经细胞核内出现特征性阳性颗粒 ,为细胞核内DNA链断裂所引起的凋亡细胞。结论 :海马神经元的迟发性死亡表现为坏死与凋亡共存现象 ,海马CA1段锥体细胞的病理改变以坏死为主 ,齿状回颗粒细胞的改变以凋亡为主。对于迟发性神经元死亡的病因 ,生物学过程 ,及与神经系统退行性变的关系进行了初步探讨。Objective:To observe the morphological changes of delayed neuronal death in cerebral hippocampal sections of mice with repeated ischemia-reperfusion. Methods: We made incomplete cerebral ischemia-reperfusion modern through clipping bilateral common carotid arteries repeatedly. At 24 hours,72 hours and 7days After ischemia-reperfusion, we collected mice brains and made sections. These sections were stained by HE, and by the method of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL). Results: At 7 days after reperfusion, there appeared diffuse necrosis in hippocampal pyramidal neurons, it is most obvious in CA1 region. By in situ end-labeling of DNA, we can see particular positive particles in some cellular nucleus of granule neurons in dentate gyrus, of pyramidal neurons in the regions of CA2, CA3, and of some neurons in CA1 nearby necrosis neurons. It would be apoptotic cells. Conclusions: Delayed neuronal death existed as two forms of apoptosis and necrosis in the hippocampal sections, necrosis is the main pathological alteration at CA1 pyramidal neurons, whereas apoptosis is obvious at granule neurons of dentate gyrus. The pathogenesis and biological process of delayed neuronal death and its relationship with degeneration of nervous system had been discussed preliminarily.

关 键 词：迟发性神经元死亡 海马 动物模型 脑缺血 再灌注损伤 

分 类 号：R743[医药卫生—神经病学与精神病学]