大肠癌组织Lewis X,唾液酸化Lewis X,Lewis A和唾液酸化Lewis A的表达及其意义  

Expression of LeX ,Sialyl-LeX, LeA and Sialyl-LeA in Colorectal Carcinoma and Its Significance

作　　者：尚培中[1] 谷化平[1] 孙印臣[1] 王佩玲[1] 卢育奇[1] 刘景章[1] 李世存[1] 

机构地区：[1]解放军第251医院普通外科,河北张家口075000

出　　处：《中国医师杂志》2002年第11期1207-1209,共3页Journal of Chinese Physician

摘　　要：目的　研究LewisX (LeX) ,唾液酸化LewisX (sialyl-LeX) ,LewisA (LeA)和唾液酸化LewisA (sialyl-LeA)在大肠癌中的表达状况及其与肿瘤发生、分化、转移及预后的关系。方法　应用催化信号放大法 (CSA)检测 90例大肠癌、5 1例转移淋巴结和 30例远离癌组织的正常大肠黏膜LeX ,sialyl-LeX ,LeA和sialyl-LeA的表达状况 ,并对其中 5 3例患者进行随访。结果　LeX ,sialyl -LeX ,LeA和sialyl-LeA阳性物质在远离癌组织的正常大肠黏膜中阳性表达率分别为 93 3% (2 8/ 30 ) ,16 7% (5 / 30 ) ,93 3% (2 8/ 30 )和 90 0 % (2 7/ 30 ) ,而在大肠癌组织中阳性率分别为 90 0 % (81/ 90 ) ,94 4 % (85 / 90 ) ,78 9% (71/ 90 )和 80 0 % (72 / 90 ) ,转移淋巴结标本中阳性率分别为 6 8 7% (35 / 5 1) ,96 1% (49/ 5 1) ,6 6 7% (34/ 5 1)和 6 6 7% (34/ 5 1)。结论　癌细胞膜Lewis糖抗原的唾液酸化及异常糖基的形成 ,可能与肿瘤转移行为有关。sialyl-LeX可以作为预测大肠癌侵袭、转移的肿瘤相关抗原 ,对评估患者预后较LeX ,LeA和sialyl-LeA具有更重要的临床意义。Objective To investigate the level of cell adhesion molecules LeX , sialyl-LeX, LeA and sialyl-LeA expression in colorectal carcinoma and its relation with carcinogenesis, differentiation, metastasis and prognosis.Methods The expression forms of LeX,sialyl-LeX,LeA and sialyl-LeA were studied by immunohistochemistry method with catalysis signal amplification (CSA)in 90 carcinomas and 30 normal mucosal specimens of colon and rectum, as well as in 51 metastatic lymph nodes.Fifty-three patients were followed up. Results Their antigens were expressed in normal mucosa distant from carcinoma lesions with the following frequencies: LeX, 93 3% (28/30); sialyl-LeX, 16 7% (5/30); LeA, 93 3% (28/30); and sialyl-LeA, 90 0% (27/30), whereas in colorectal carcinomas, the expression frequency rate were: LeX, 90 0% (81/90); sialyl-LeX, 94 4% (85/90); LeA, 78 9% (71/90); and sialyl-LeA, 80 0%(72/90). In metastatic lesion of lymphode LeX, sialyl-LeX, LeA and sialyl-LeA were expressed in 68 7% (35/51),96 1% (49/51),66 7% (34/51) and 66 7% (34/51), respectively.Conclusions The alteration of Lewis-related carbohydrate antigens in cancer cell membranes, including sialylation and/or aberrant glycosylation,these alteration may be related to metastatic behavior. These results suggested that sialyl-LeX may be used for estimating infiltration, metastasis and prognosis as a tumor-associated antigen in colorectal carcinoma.

关 键 词：大肠癌 唾液酸化 大肠肿瘤 细胞粘附分子 sialyl-LeX抗原 免疫组织化学 肿瘤标志物 Lewis糖抗原 

分 类 号：R735.34[医药卫生—肿瘤]