大鼠延髓背角内GABA或甘氨酸免疫阳性终末与Fos阳性投射神经元的联系  

CONNECTIONS BETWEEN GABA-OR GLYCINE-IMMUNOREACTIVE TERMINALS AND Fos-POSITIVE PROJECTION NEURONS IN THE RAT MEDULLARY DORSAL HORN

作　　者：李辉[1] 李云庆[1] 

机构地区：[1]第四军医大学解剖学教研室暨梁琚脑研究中心,西安710032

出　　处：《解剖学报》2002年第6期577-580,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (3 9970 2 3 93 980 0 0 443 980 0 0 3 4);国家重点基础研究规划"脑功能和脑重大疾病的基础研究"(G19990 5 40 0 0 );高等学校骨干教师资助计划

摘　　要：目的　观察大鼠延髓背角 (MDH)内向丘脑或臂旁核投射的Fos阳性神经元与γ 氨基丁酸 (GABA)或甘氨酸 (Gly)阳性终末的联系。　方法　四甲基罗达明 (TMR)逆行追踪结合TMR、Fos、GABA或Gly的免疫荧光三重染色技术。　结果　GABA或Gly阳性终末主要分布于延髓背角浅层 (Ⅰ、Ⅱ层 ) ;给予面口部痛刺激后 ,Fos阳性神经元也主要分布于浅层 ;TMR注入一侧丘脑或臂旁核 ,逆标神经元分别主要见于对侧或同侧MDH的浅层 ;部分逆标神经元呈Fos阳性 ;GABA或Gly阳性终末与Fos阳性投射神经元形成密切接触。　结论　MDH内感受面口部伤害性刺激信息的部分神经元向丘脑或臂旁核投射 ,GABA或Gly可能对这些伤害性感受神经元发挥抑制作用。Objective To investigate the connections between γ\|aminobutyric acid(GABA)\|or glycine (Gly)\|containing terminals and Fos\|positive projection neurons from the medullary dorsal horn (MDH) to the thalamus or parabrachial nuclei(PBN). Methods Tetramethyl rhodamine(TMR) retrograde tracing,combined with immunofluorescence histochemical triple\|staining for TMR/Fos/GABA or TMR/Fos/Gly was used. Results GABA\|or Gly\|immunoreactive terminals were chiefly located in the superficial laminae(laminae Ⅰ and Ⅱ) of the MDH.After orofacial noxious stimulation.Fos\|positive neurons were also mainly observed in the superficial laminae.After injecting TMR into the unilateral thalamus or PBN,TMR retrogradely labeled neurons were mainly distributed in the superficial laminae of the controlateral or ipsilateral MDH,respectively.Some of these TMR\|labeled neurons also exhibited Fos\|immunoreactivities.GABA\| or Gly\|containing terminals made close contacts with Fos positive retrogradely labeled neurons.Conclusion\ In the superficial laminae of the MDH,some of the orofacial nociceptive neurons send project fibers directly to the thalamus or PBN,GABA and Gly might exert their inhibitory effects on these nociceptive projection neurons.\;[

关 键 词：延髓背角 神经元 γ-氨基丁酸 甘氨酸 伤害性感受 FOS 大鼠 

分 类 号：R338.1[医药卫生—人体生理学]