短暂激活c-jun N-terminal激酶抑制胆红素诱导的SH-SY5Y细胞凋亡  

Transient activation of c-jun N-terminal kinase inhibits bilirubin-induced apoptosis of SH-SY5Y cells

作　　者：韩张[1] 胡萍[2] 倪道凤[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院耳鼻咽喉科,北京100730 [2]中国医学科学院中国协和医科大学基础医学研究所药理室,北京10005

出　　处：《基础医学与临床》2002年第5期473-476,共4页Basic and Clinical Medicine

摘　　要：探讨c junN terminal激酶 (JNK)在胆红素诱导人神经母细胞瘤SH SY5Y细胞凋亡信号转导中的作用。我们用 30 0 μmol LH2 O2 预处理SH SY5Y细胞 1h以激活JNK ,与经PBS预处理的对照组比较 ,流式细胞仪结果显示凋亡细胞百分比由 (37 4± 3 2 ) %降低到 (12 6± 2 6 ) %。为了进一步证实JNK的作用 ,在SH SY5Y细胞经H2 O2 预处理前 ,先经 2 0 μmol LJNK c jun AP1抑制剂curcumin作用 12h以抑制H2 O2 对JNK的激活 ,流式细胞仪结果显示凋亡细胞增高到 (42 8± 4 4 ) % ,curcumin完全抑制了短暂激活JNK所提供的保护作用。结果表明短暂激活JNK可能在胆红素诱导的SH SY5Y细胞凋亡中提供抗凋亡信号 。To investigate the role of c jun N terminal kinase(JNK) in bilirubin induced apoptosis of human neuroblastoma SH SY5Y cells, JNK was activated by pretreatment with 300μmol/L H 2O 2 for 1 hour. Compared with control pretreated with PBS, flow cytometry showed that the number of apoptotic cells decreased dramatically from (37.4±3.2)% to (12.6±2.6)% in SH SY5Y cells pretreated with H 2O 2. To further identify the role of JNK, we used curcumin to inhibit the activation of JNK by H 2O 2 . Flow cytometry showed that curcumin totally inhibited the protective effect of transient activation of JNK on bilirubin induced apoptosis in SH SY5Y cells. The results indicated the transient activation of JNK functioned as a survival signal for bilirubin induced apoptosis in SH SY5Y cells.

关 键 词：细胞凋亡 胆红素 JNK 人神经母细胞瘤细胞 

分 类 号：R363[医药卫生—病理学]