^(125)I-脱氧尿嘧啶核苷对肝癌细胞HepG2生长的抑制作用  

Inhibition effects of ~ (125)I-UdR on the growth of HepG2 cells

作　　者：张海涛[1] 张志德[1] 李金泉[2] 

机构地区：[1]苏州大学附属第一医院普外科,215006 [2]苏州大学附属第一医院神经外科,215006

出　　处：《中华核医学杂志》2002年第5期286-288,共3页Chinese Journal of Nuclear Medicine

摘　　要：目的　评价12 5 I 脱氧尿嘧啶核苷 (UdR)对肝癌细胞HepG2生长的抑制作用及影响因素。方法　HepG2细胞在含有12 5 I UdR培养基中培养后测量其放射性 ,观察HepG2细胞对12 5 I UdR的摄取 ;用细胞克隆形成法评价12 5 I UdR对HepG2细胞生长的抑制作用。结果　HepG2摄取12 5 I UdR量随培养基中12 5 I UdR浓度增加而增加 ,两者显著相关 (r =0 .99)。HepG2对12 5 I UdR的摄取明显高于Na12 5 I。HepG2细胞摄取12 5 I UdR后其生长受到抑制 ,两者呈明显负相关 (r =- 0 .94 3) ,半数致死剂量(LD5 0 )为 ( 0 .87± 0 .2 9)kBq/mL。12 5 I UdR组细胞存活分数明显低于Na12 5 I组。结论　12 5 I UdR对HepG2细胞生长有明显抑制作用 ,HepG2细胞摄取12 5 I UdR量有浓度依赖性。Objective To determine the inhibition effects of ~ 125I-UdR on the growth of human hepatoma cell line HepG2 and the influencing factors. Methods ~ 125I-UdR was added to the HepG2 culture medium. The amount of ~ 125I-UdR uptake by HepG2 cells was evaluated through measuring the radioactivity per cell; the inhibiting effects of ~ 125I-UdR on HepG2 growth were estimated by clonal forming method. Results The amounts of ~ 125I-UdR uptaken by HepG2 increased with the dose of ~ 125I-UdR escalating in the culture medium (r=0.99). The uptake of ~ 125I-UdR by HepG2 cells was higher than that of Na~ 125I. As the ~ 125I-UdR concentration in HepG2 escalating, the inhibition effects became stronger (r=-0.943) and the LD 50 was (0.87±0.29) kBq/mL. The survival fraction of ~ 125I-UdR group was significantly lower than that of Na~ 125I group. Conclusions The inhibition effects of ~ 125I-UdR on HepG2 cell growth were obvious. ~ 125I-UdR may be incorporated inside the HepG2 cells and the uptake of HepG2 was dose dependent.

关 键 词：肝肿瘤 放射疗法 脱氧尿苷 碘放射性同位素 

分 类 号：R735.7[医药卫生—肿瘤] R730.[医药卫生—临床医学]