浙江蝮蛇毒诱导人白血病Jurkat细胞凋亡的体外实验研究  

Effect of Snake Venom of Agkistrodon Halys Pallas in Inducing Human Leukemic Jurkat Cell Apoptosis

作　　者：董庆华[1] 郑树[1] 吕庆华[1] 何立明[1] 

机构地区：[1]浙江大学附属第二医院肿瘤研究所,杭州310009

出　　处：《中国中西医结合杂志》2002年第11期851-853,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省卫生厅资助课题 (No .2 0 0 2A1 0 6);浙江省科委资助课题(No.2 0 0 2D30 0 7)

摘　　要：目的 :研究浙江蝮蛇毒诱导人白血病Jurkat细胞凋亡的作用及机理。方法 :采用MTT法测定IC50 值及生长曲线 ,流式细胞仪 (FCM )AnnexinⅤFITC/PI法检测细胞凋亡发生率 ,PI染色法检测细胞周期 ,同时用流式细胞汁FCM及Western blot检测Bcl 2蛋白表达。结果 :浙江蝮蛇毒能抑制Jurkat细胞生长 ,且呈剂量依赖关系 ,并能诱导人白血病Jurkat细胞凋亡 ,4 8h时作用最强 ,此时Bcl 2蛋白表达下降 ,随后此作用减弱 ,低浓度作用下细胞逐渐恢复生长。结论 :浙江蝮蛇毒能诱导人白血病Jurkat细胞凋亡 ,且呈剂量依赖关系 ,此作用与BclObjective: To study the effect and mechanism of snake venom of Agkistrodon Halys Pallas in Zhejiang in inducing human leukemic Jurkat cell apoptosis. Methods: IC 50 and growth curve of Jurkat cells were detected by MTT; percentage of apoptotic cells was analyzed by flow cytometry (FCM) using Annexin Ⅴ FITC/PI staining method; cell cycle was also detected by FCM and protein expression of Bcl 2 gene was detected by FCM and Western blot. Results: Zhejiang viper venom could inhibit the growth of Jurkat cells dose dependently, induce the cell apoptosis, with the Bcl 2 protein expression down regulated. The strongest effect was shown at 48 hrs, it weakened gradually, and the Jurkat cells restored slowly in low concentration of snake venom. Conclusion: Zhejiang viper venom could induce apoptosis of human leukemic Jurkat cell in dose dependent manner, the effect is related to the down regulation of Bcl 2 protein expression.

关 键 词：浙江蝮蛇毒 白血病 JURKAT细胞 BCL-2蛋白表达 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]