残肾大鼠血小板反应蛋白表达与肾组织纤溶状态的关系  

Relationship between thrombospondin-1 expression and urokinase-typed activator expression and significance on fibrinolytic system in rats with remnant kidneys

作　　者：王翠玲[1] 马宏[1] 李晓惠[1] 李钊[1] 刘源[1] 郝翼忠[1] 

机构地区：[1]山西医科大学第一临床学院儿科,030001

出　　处：《中国药物与临床》2002年第6期354-357,共4页Chinese Remedies & Clinics

基　　金：山西省自然科学基金资助项目 (2 0 0 2 110 4)

摘　　要：目的　研究 7/ 8肾切除慢性肾衰大鼠肾小管间质病变过程中血小板反应蛋白 (TSP) 1与尿激酶型纤溶酶原激活物 (uPA)蛋白表达在调节纤溶系统功能中的意义。方法　以 7/ 8肾切除SD大鼠为实验动物模型 ,予水蛭和ACEI 洛汀新治疗 12周后杀检残肾常规病理改变 (PAS染色 )。免疫组织化学染色观察残肾组织TSP 1与uPA蛋白表达变化 ,并比较二者表达的相关性及TSP 1与间质纤维化病变的相关性。结果　 7/ 8肾切除后大鼠残肾出现进行性肾小球硬化及间质纤维化病变 ,临床上表现为慢性肾功能衰竭。给予治疗后肾功能明显改善 ,残肾组织病理改变好转。免疫组化提示肾小管间质区TSP 1、uPA蛋白表达上调 ,且二者表达在时相和定位上呈正相关 ,而TSP 1表达与间质纤维化程度则呈负相关。结论　本研究提示在慢性肾衰大鼠间质病变经治疗恢复过程中uPA与TSPObjective To study the modulating action on the fibrinolytic system,of thrombospondin 1 (TSP 1) and urokinase typed plasminogen activator (uPA) that express in remnant renal tissues of 7/8 nephrectomized rats during their tubulointerstitial fibrotic lesions.Methods 7/8 nephrectomized SD rats served as experimental animal model,After treated for 12 weeks with combination of Hirudo and Lotensin,rats were sacrificed and renal morphological changes were detected by use of PAS staining.The protein expression of TSP 1 and uPA in areas of remnant renal tubulointerstitium,the correlation between both proteins and TSP 1 with tubulointerstitial fibrotic lesions were estimated by means of immunohistochemical technique.Results The progressive glomerulosclerosis,tubulointerstitial fibrosis and chronic renal failure developed gradually after 7/8 nephrectomy in rats.But following the treatment,the renal functional parameters and pathological lesions ameliorated more significantly than untreated group.The immunostaining suggested that the expression of TSP 1 and uPA in the regions of tubulointerstitium was up regulated,and these two proteins′expression had showed a positive correlation on a time location dependent pattern,but a negative correlation had presented between TSP 1 and tubulointerstitial lesions.Conclusion The expression of TSP 1 and uPA in remnant renal tissues may be play an importment role in enhancing the fibrinolytic function during the restoration stage of tubulointerstitial lesions after treatment.

关 键 词：残肾 大鼠 血小板反应蛋白 肾组织 尿激酶型纤溶酶原激活物 肾小管间质病变 

分 类 号：R363[医药卫生—病理学]