与肿瘤侵袭相关的Lewis抗原在人肝癌细胞H7721上的表达及其合成的酶学分析  

Expression of tumor-invasion-related Lewis antigens on the surface of H7721 human hepatocarcinoma cells and the enzymatic analysis of their synthesis

作　　者：郭鹏[1] 张英 张夏英[1] 陈惠黎[1] 张延 成松久 

机构地区：[1]复旦大学医学院生化教研室卫生部糖复合物重点实验室,上海200030 [2]产业技术综合研究所,分子细胞工学部门,日本筑波3058568

出　　处：《肿瘤》2002年第6期448-452,共5页Tumor

摘　　要：目的　研究人肝癌细胞H772 1表面 4种含岩藻糖类抗原—Lewis抗原的表达、它们与肝癌细胞在体外侵袭行为的关系 ,及其合成相关酶α1,3岩藻糖转移酶 (FucT) 5种亚型的基因表达。方法　用单克隆抗体结合流式细胞仪测定Lewis抗原 ,用涂有Matrigel膜的转移小室测定细胞的侵袭行为 ,利用实时RT PCR测定FucT的表达。结果　H772 1细胞表面主要表达Slex和少量SDLex,而Lex和Slea表达极微。其中只有Slex和H772 1细胞的侵袭明显相关。H772 1细胞中FucT的表达强度为FucT IV >FucT III >FucT VI≥FucT VII,后两者表达少 ,而几乎完全缺乏FucT IX。结论　Slex是H772 1细胞表面最多也是与细胞侵袭最有关的Lewis抗原 ,因据报道FucT VI催化合成Slex和SDLex的效率高于FucT III及VII,故可能是负责H772 1细胞中Slex和SDLex合成的主要FucT ,但也不排除FucT VII和FucT III也参与Slex的合成。合成Lex中最重要的FucT IX的缺乏可能是Lex低表达的原因。Objective The expression of four fucose containing sugar antigens, Lewis antigens, on the surface of H7721 human hepatocarcinoma cells, their relations to the invasion of hepatocarcinoma cells ex vivo and the gene expresssion of the five subtypes of α1,3 fucosyltransferase (FucT) responsible for the synthesis of Lewis antigens were studied. Methods The Lewis antigens were detected with monoclonal antibodies combined with flow cytometry. The invasion abilities were assayed by using transwells coated with matrigel. The expressions of FucTs were determined by using real time reverse transcriptase polymerase chain reaction (RT PCR). Results It was found that H7721 cells mainly expressed SLe x and a little amount of SDLe x on the cell surface, but those of Le x and SLe a were only trace. Among them, only SLe x was related to the invasion of H7721 cells. The intensities of FucT expressions were FucT IV > FucT III > FucT VI ≥ FucT VII, the latter two were expressed very low, while FucT IX was not expressed. Conclusion SLe x is the most abundant and invasion related Lewis antigen on H7721 cell surface. Owing to the reported finding that the efficiency of FucT VI in the synthesis of SLe x and SDLe x was higher than that of FucT III and FucT VII, therefore, FucT VI is supposed to be the main enzyme responsible for the synthesis of SLe x and SDLe x , but the participation of FucT VII and FucT III in the synthesis of SLe x can not be ruled out. The absence of FucT IX, which is most important in the synthesis of Le x , may be the reason of the trace expression of Le x .

关 键 词：Lewis抗原 人肝癌细胞株 α1 3岩藻糖转移酶 肿瘤侵袭 

分 类 号：R735.7[医药卫生—肿瘤]