薏苡仁酯诱导人宫颈癌HeLa细胞凋亡的实验研究  

The mechanism of coixenolide-induced apoptosis in human cervical cancer cells

作　　者：韩苏夏[1] 朱青[2] 杜蓓茹[1] 杜兰[1] 

机构地区：[1]西安交通大学第一医院肿瘤科,西安710061 [2]陕西省人民医院肿瘤科

出　　处：《肿瘤》2002年第6期481-482,共2页Tumor

摘　　要：目的　探讨薏苡仁酯诱导人宫颈癌HeLa细胞凋亡的作用机制。方法　应用形态学方法 ,流式细胞术 (FACS) ,DNA凝胶电泳等方法检测细胞凋亡的发生 ,应用RT PCR检测凋亡相关基因Fas与FasL的变化。结果　薏苡仁酯对人宫颈癌HeLa细胞的生长有明显的抑制作用 ,并诱导肿瘤细胞发生凋亡。凋亡细胞表现为细胞固缩 ,核染色质碎裂 ,DNA凝胶电泳显示清晰的DNA梯形条带 ,FACS检测到凋亡率最高为 13%。AnnexinV标记的方法检测凋亡时发现 ,坏死与凋亡共存。在薏苡仁酯诱导人宫颈癌HeLa细胞凋亡过程中 ,凋亡相关基因Fas转录水平比用药前增强 ,而FasL转录水平减低。结论　除了坏死 ,凋亡也为薏苡仁酯抑癌的机制之一。薏苡仁酯诱导人宫颈癌HeLa细胞凋亡可能与Fas基因与FasL基因表达有关。Objective To study the mechanism of coixenolide induced apoptosis. Methods Morphological study, DNA gel electrophoresis, flow cytometry and fluorescin labeled Annexin V to detect apoptosis, RT PCR to detect the gene related with apoptosis. Results Human cervical cancer HeLa cells treated with coixenolide induced cell cycle arrest at G 2M phase, leading to apoptosis. The morphology of HeLa cells showed nuclear chromatine condensation and fragmentation. Typical ladder pattern of DNA fragmentation was observed. Transcription of Fas gene was enhanced, but FasL gene was lowered. Annexin labeling results revealed the co existense of cell apoptosis and necrosis in coixenolide treated HeLa cells. Conclusion Coixenolide induces apoptosis and necrosis of human cervical cancer. The inducton of apoptosis may berelated to expression of Fas and FasL.

关 键 词：薏苡仁酯 宫颈癌 HELA细胞 细胞凋亡 实验研究 FAS基因 FASL基因 

分 类 号：R737.33[医药卫生—肿瘤] R73－[医药卫生—临床医学]