人脑红蛋白(NGB)全长cDNA序列的克隆  

Full-length cDNA Cloning of Human Neuroglobin by Using Electronic Sequence Elongation Technique and RACE Technique

作　　者：王春丽[1] 张成岗[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《生物化学与生物物理进展》2002年第6期946-951,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 (3 0 10 0 0 493 990 0 0 413 990 0 0 74);军事医学科学院科技创新研究启动基金 (0 10 2 0 0 1990 5 10 5 )部分资助项目~~

摘　　要：人脑红蛋白 (neuroglobin ,NGB)是新发现的神经系统特异的携氧蛋白 ,然而其全长cDNA序列一直未见报道 .采用电子序列延伸技术和cDNA序列末端快速扩增技术 (rapidamplificationofcDNAends ,RACE)研究发现 ,人NGB全长cDNA序列为 190 9bp,5′非编码区为 375bp ,编码区 (45 6bp)可编码 15 1个氨基酸 ,3′非编码区为 10 78bp,其中含 2 7bp的 poly (A) (GenBank接受号 :AF4 2 2 797) .综合采用电子序列延伸技术与RACE技术是获得全长cDNA序列的有效方法 ,为后续的功能研究提供了重要基础 .Human neuroglobin (NGB) is a newly discovered gene functioned specifically in the oxygen supply and oxygen consumption in the brain. However, the full length cDNA sequence of NGB was not reported yet. Using the in silicon sequence elongation technique, 5′ RACE and 3′ RACE technique, the full length cDNA sequence of human neuroglobin was successfully obtained (GenBank accession number AF422797). The cDNA sequence of human NGB is 1 909 bp in size and capable of encoding a protein of 151 amino acids. The 5′ untranslated region is 375 bp, while the 3′ untranslated region is 1 078 bp including 27 bp of poly(A) sequence. Results demonstrated that the RACE technique coupled with electronic sequence elongation technique is useful to obtain unknown sequence of 5′ terminal or 3′ terminal part of a new gene.

关 键 词：人脑红蛋白 全长CDNA序列 克隆 电子序列延伸技术 CDNA末端快速扩增技术 

分 类 号：Q513.4[生物学—生物化学] Q78