喉癌STK15基因表达和染色体不稳定的研究  

Expression of STK15 Gene and Chromosomal Instability in Laryngeal Carcinoma

作　　者：李英慧[1] 李福才[1] 赵旭[1] 赵震[1] 孙开来[1] 孙兴和 

机构地区：[1]中国医科大学医学遗传学教研究,沈阳110001 [2]空军463医院耳鼻喉科,沈阳110042

出　　处：《Acta Genetica Sinica》2002年第12期1048-1051,共4页

基　　金：辽宁省自然科学基金 (批准号 :2 0 0 110 10 3 9) ;国家自然科学基金 (批准号 :3 0 1710 0 8)资助

摘　　要：为研究喉鳞状细胞癌中STK15基因表达及其与染色体不稳定的相关性 ,提取 5 0例喉鳞状细胞癌及配对癌旁正常组织和人喉鳞状细胞癌Hep - 2细胞系的RNA ,反转录合成cDNA ,以 β -actin为内对照进行PCR扩增 ,用软件分析电泳结果 ,研究喉癌中STK15基因表达的水平 ;以Hep - 2细胞系为代表应用常规和高分辨G显带方法进行核型分析。在 5 0例喉癌中 ,癌组织STK15表达高于配对癌旁正常组织的有 34例 ,占 6 8% ,经统计学分析 ,肿瘤组与对照组差异显著 ,Hep - 2细胞系中STK15基因表达高于内对照 β-actin ;Hep - 2细胞系中存在显著的染色体不稳定 :染色体数目变化于 4 3～ 84条之间 ,众数为 6 9～ 74条 ,结构畸变主要表现为 13条标记染色体。本研究首次发现STK15基因在喉癌中表达增高 ,它可能通过中心体异常而引起染色体不稳定 ,在喉癌的发生、发展中发挥一定作用。To assess the relationship between expression of STK15 gene and chromosomal instability in laryngeal squamous cell carcinoma,RNA was extracted from 50 cases of laryngeal squamous cell carcinoma and paired normal tissue and Hep-2 cell line. cDNA was synthesized through reverse transcription,which was amplified by PCR using β actin as contrast. The results of electrophoresis were analysed by software to examine the expression level of STK15 gene in laryngeal carcinoma;karyotype analysis of Hep-2 cell line as an example was performed by routine and high resolution G banding techniques. In the 50 cases of laryngeal carcinoma,there were 34 cases whose expression of STK15 gene in tumor was higher than paired normal tissue,occupying 68%. The difference between tumor group and contrast group was prominent by statistic analysis. The expression of STK15 gene in Hep-2 cell line was higher than that of β -actin;Chromosomal instability in Hep-2 cell line was evident: The chromosomal number ranged from 43 to 84 and the chromosomal model ranged from 69 to 74. The structural abnormality was represented by 13 marker chromosomes. We discovered the overexpression of STK15 gene in laryngeal carcinoma the first time. It may caused chromosomal instability through abnormal centrosome, therefore having some effect during the occurrence and development of laryngeal carcinoma.

关 键 词：喉癌 STK15基因 表达 染色体不稳定 喉鳞状细胞癌 中心体 HEP-2细胞 

分 类 号：R739.65[医药卫生—肿瘤]