Pepsinogen C Gene Expression in Epithelial Cells of Rat Stomach During Development  

胃蛋白酶C基因在大鼠胃底腺发育过程中上皮细胞内的表达(英文)

作　　者：戈应滨[1] 

机构地区：[1]南京医科大学生理学系,南京210029

出　　处：《Developmental and Reproductive Biology》2002年第2期88-94,共7页发育与生殖生物学报（英文版）

基　　金：TheprojectwassupportedbyScienceandTechnologyGrantofNanjingMedicalUniversity(No.NY980 0 3 )

摘　　要：Pepsinogens are zymogens of pepsins,aspecific proteases working as digestive enzymes in vertebrate stomach,of which biological and molecular properties have been extensively studied.Several exhaustive studies have been performed in the pepsinogen producing cells in developing rat stomachs,but little is known about the expression of pepsinogen gene in these cells.In this study,the ontogeny of pepsinogen producing cells in rat fundic glands was studied by in situ hybridization using a digoxigenin-labeled RNA probe.The rat gastric epithelium was stratified but was morphologically undifferentiated at the stage of 18.5 days of gestation.The pepsinogen mRNA was expressed both in chief cells and mucous neck cells in adult rats,which was first detected by in situ hybridization in the stomach of the rats at 3.5 days after birth.The development of pepsinogen producing cells could be classified into four stages:(1) 18.5 days of gestation to 0.5 day after birth;(2) 3.5 days to 2 weeks after birth;(3) 3～4 weeks after birth;(4) 8 weeks after birth.Pepsinogen expression is strictly limited to these cells,the distribution of which shown a developmental stage-specific manner.We concluded the pepsinogen C could offer excellent molecular markers of differentiation during stomach epithelial cellulur development.胃蛋白酶原是胃蛋白酶的前体 ,是脊椎动物中普遍存在的非特异性蛋白消化酶 ,胃蛋白酶的生物学特性及分子特性已得到了充分研究。虽然有许多关于胃蛋白酶产生细胞的研究报告 ,但胃蛋白酶原基因在这些细胞的表达却知之甚少。本研究采用地高辛标记的RNA探针 ,用原位杂交的方法研究了大鼠胃底腺中胃蛋白酶产生细胞的个体发育。研究发现 ,大鼠胃腺在胚胎 1 8.5天开始出现 ,但没有形态分化 ;胃蛋白酶的mRNA在出生后 3.5天首次被原位杂交法检出。胃蛋白酶产生细胞在出生后 8周发育成熟 ,胃蛋白酶的mRNA表达在主细胞和颈粘液细胞内 ,其发育可分为 4个阶段 :( 1 )胚胎 1 8.5天至出生后 0 .5天 ;( 2 )出生后 3.5天至 2周 ;( 3)出生后 3周至 4周 ;( 4 )出生后 8周。在胃底腺发育过程中 ,胃蛋白酶mRNA的表达只局限于某种特定的细胞 ,这些细胞的分布具有明确的阶段特异性。因此 ,我们认为胃蛋白酶C可作为胃上皮细胞分化的分子标志。

关 键 词：pepsinogen C ONTOGENY gene expression fundic gland in situ   hybridization RAT 

分 类 号：Q556.9[生物学—生物化学] Q482